Human/Mouse/Rat SH2B1 Antibody

Catalog #: AF6915 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF6915
AF6915-SP

Detection of Human, Mouse, and Rat SH2B1 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat SH2B1 Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human, mouse, and rat SH2B1 in Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human SH2B1
Pro317-Leu467
Accession # Q9NRF2

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human, Mouse, and Rat SH2B1 by Western Blot. Western blot shows lysates of LNCaP human prostate cancer cell line, Neuro-2A mouse neuroblastoma cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat SH2B1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6915) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for SH2B1 at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SH2B1

SH2B1 (Src-Homology 2 Domain-Containing protein B1/1B; also SH2B1 alpha, PSM and SH2B adaptor protein 1) is a member of the SH2B adapter family of proteins. Although its predicted MW is 80 kDa, it runs anomalously at 90-102 kDa in SDS-Page. SH2B1 is a nucleocytoplasmic protein that is widely expressed in cells such as skeletal muscle cells, neurons, and adipocytes. It serves as a linker between Jak2 and multiple downstream molecules such as Rac and IRS1. It does this following phosphorylation on a number of potential sites. It also promotes the enzymatic activity of associated receptor kinases, thus potentiating ligand-receptor interactions. SH2B1 appears to bind to nonphosphorylated Jaks as a monomer, while Jak phosphorylation induces SH2B1 dimerization. SH2B1 is further reported to form a homopentamer, and to oligomerize with SH2B2. Human SH2B1( alpha ) is 756 amino acids amino acids (aa) in length. It contains one dimerization segment (aa 24-85), an NLS (aa 224-233), one pleckstrin homology region (aa 249-378) and an SH2 domain (aa 521-625). At least three utilized phosphorylation sites exist. There are at least two splice variants for SH2B1. The 90-92 kDa SH2B1 beta isoform shows a 39 aa substitution for aa 633-756, while the SH2B1 gamma isoform possesses a 50 aa substitution for the same aa range (i.e., aa 633-756). Over aa 317-467, human SH2B1 shares 92% aa identity with mouse SH2B1.

Long Name

SH2B adaptor protein 1

Entrez Gene IDs

25970 (Human); 20399 (Mouse)

Alternate Names

FLJ30542; KIAA1299; Pro-rich, PH and SH2 domain-containing signaling mediator; PSM; SH2 domain-containing protein 1B; SH2 domain-containing putative adapter SH2-B; SH2B adapter protein 1; SH2B adaptor protein 1; SH2-B signaling protein; SH2B1; SH2BDKFZp547G1110