Home / Vang-like Protein 2/VANGL2 / Human/Mouse/Rat Vang-like Protein 2/VANGL2 Antibody

Human/Mouse/Rat Vang-like Protein 2/VANGL2 Antibody

Catalog #: AF4815
12 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF4815
AF4815-SP
Detection of Human/Mouse/Rat Vang-like Protein 2/ VANGL2 by Western Blot.
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Citations (12)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat Vang-like Protein 2/VANGL2 Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human, mouse, and rat Vang-like Protein 2/VANGL2 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human VANGL1 is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human Vang-like Protein 2/VANGL2
Gln241-Val521
Accession # Q9ULK5
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human/Mouse/Rat Vang-like Protein 2/ VANGL2 antibody by Western Blot. View Larger

Detection of Human/Mouse/Rat Vang-like Protein 2/ VANGL2 by Western Blot. Western blot shows lysates of mouse kidney, human and rat brain tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse/Rat Vang-like Protein 2/VANGL2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4815) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Vang-like Protein 2/VANGL2 at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Immunohistochemistry Vang-like Protein 2/VANGL2 antibody in Mouse Embryo by Immunohistochemistry (IHC-Fr). View Larger

Vang-like Protein 2/VANGL2 in Mouse Embryo. Vang-like Protein 2/VANGL2 was detected in immersion fixed frozen sections of mouse embryo (E15.5) using Sheep Anti-Human/Mouse/Rat Vang-like Protein 2/VANGL2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4815) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling when primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. Specific staining was localized to developing spinal cord. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Vang-like Protein 2/VANGL2

VANGL2 (van Gogh-like protein 2; also Strabismus and Ltap) is a 65 kDa member of the Vang family of proteins. It is found in the membranes of embryonic neurons and epithelium where it associates with DVL1-3 and various frizzleds. VANGL2 mutations are associated with neural tube defects. Human VANGL2 is a 521 amino acid (aa) 4-transmembrane protein that contains an N-terminal (aa 1‑108) and C-terminal (aa 239‑521) cytoplasmic domain. There is a Ser-rich region in the N-terminus (aa 5‑20) and a PDZ-binding motif over aa 506‑521. Over aa 241‑521, human, mouse and rat show total identity in amino acid sequence.

Entrez Gene IDs
57216 (Human); 93840 (Mouse); 289229 (Rat)
Alternate Names
KIAA1215Loop-tail protein 1 homolog; loop-tail-associated protein; LPP1; LTAP; LTAPVan Gogh-like protein 2; MGC119403; MGC119404; STB1; STB1Strabismus 1; STBM; STBM1; Strabismus; vang (van gogh, Drosophila)-like 2, vang, van gogh-like 2 (Drosophila); Vang like Protein 2; VANGL2; vang-like 2 (van gogh, Drosophila); Vang-like Protein 2

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Citations for Human/Mouse/Rat Vang-like Protein 2/VANGL2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

12 Citations: Showing 1 - 10
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  1. Phosphatidylinositol transfer protein/planar cell polarity axis regulates neocortical morphogenesis by supporting interkinetic nuclear migration
    Authors: Xie Z, Bankaitis VA
    Cell reports
  2. Rab11a Is Essential for the Development and Integrity of the Stereocilia and Kinocilia in the Mammalian Organ of Corti
    Authors: Lindsey Knapp, Haojie Sun, Yan-Mei Wang, Bin-Jun Chen, Xi Lin, Nan Gao et al.
    eNeuro
  3. Rab11a Regulates the Development of Cilia and Establishment of Planar Cell Polarity in Mammalian Vestibular Hair Cells
    Authors: Bin-Jun Chen, Xiao-qing Qian, Xiao-yu Yang, Tao Jiang, Yan-mei Wang, Ji-han Lyu et al.
    Frontiers in Molecular Neuroscience
  4. Prickle promotes the formation and maintenance of glutamatergic synapses by stabilizing the intercellular planar cell polarity complex
    Authors: Y Ban, T Yu, B Feng, C Lorenz, X Wang, C Baker, Y Zou
    Science Advances, 2021-10-06;7(41):eabh2974.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. Non-canonical Wnt signalling regulates scarring in biliary disease via the planar cell polarity receptors
    Authors: D. H. Wilson, E. J. Jarman, R. P. Mellin, M. L. Wilson, S. H. Waddell, P. Tsokkou et al.
    Nature Communications
  6. Morphological and molecular comparisons between tibialis anterior muscle and levator veli palatini muscle: A preliminary study on their augmentation potential
    Authors: X Cheng, L Song, M Lan, B Shi, J Li
    Exp Ther Med, 2017-10-30;15(1):247-253.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  7. Cilia distribution and polarity in the epithelial lining of the mouse middle ear cavity
    Authors: W Luo, H Yi, J Taylor, JD Li, F Chi, NW Todd, X Lin, D Ren, P Chen
    Sci Rep, 2017-03-30;7(0):45870.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  8. Syndecan 4 controls lymphatic vasculature remodeling during mouse embryonic development
    Authors: Yingdi Wang, Nicolas Baeyens, Federico Corti, Keiichiro Tanaka, Jennifer S. Fang, Jiasheng Zhang et al.
    Development
  9. Planar cell polarity gene expression correlates with tumor cell viability and prognostic outcome in neuroblastoma
    Authors: C Dyberg, P Papachrist, BH Haug, H Lagercrant, P Kogner, T Ringstedt, M Wickström, JI Johnsen
    BMC Cancer, 2016-03-31;16(1):259.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  10. VANGL2 regulates membrane trafficking of MMP14 to control cell polarity and migration.
    J. Cell. Sci., 2012-02-22;125(0):2141-7.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  11. Nodal dependent differential localisation of dishevelled-2 demarcates regions of differing cell behaviour in the visceral endoderm.
    Authors: Trichas G, Joyce B, Crompton LA, Wilkins V, Clements M, Tada M, Rodriguez TA, Srinivas S
    PLoS Biol., 2011-02-22;9(2):e1001019.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  12. Vang-like protein 2 and Rac1 interact to regulate adherens junctions.
    Authors: Lindqvist M, Horn Z, Bryja V, Schulte G, Papachristou P, Ajima R, Dyberg C, Arenas E, Yamaguchi TP, Lagercrantz H, Ringstedt T
    J. Cell. Sci., 2010-01-12;123(0):472-83.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Immunoprecipitation

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