Human/Mouse/Rat VHR Antibody

Catalog #: MAB1654 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB1654
MAB1654-SP

Detection of Human, Mouse, and Rat VHR by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat VHR Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human, mouse, and rat VHR in Western blots.

Source

Monoclonal Mouse IgG_2A Clone # 237020

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human VHR
Ser2-Pro185
Accession # P51452

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

0.5 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human, Mouse, and Rat VHR by Western Blot. Western blot shows lysates of TE-85 human osteogenic sarcoma cell line, TF-1 human erythroleukemic cell line, and C2C12 mouse myoblast cell line. PVDF membrane was probed with 0.5 µg/mL Mouse Anti-Human/Mouse/ Rat VHR Monoclonal Antibody (Catalog # MAB1654) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, Recombinant Human VHR (Catalog # 1654-VH) (2 ng) was included. A specific band for VHR was detected at approximately 24 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VHR

Vaccinia Virus VH1-related Phosphatase (VHR), also known as Dual-Specificity Phosphatase 3 (DUSP3), removes inorganic phosphate groups covalently attached to tyrosine, serine and threonine residues in proteins (1). It belongs to a family of phosphatases that selectively dephosphorylate MAP kinases. VHR has been shown to dephosphorylate cellular ERK1, ERK2 and JNK, but not p38 (2, 3). Phosphorylation of VHR at tyrosine 138 by ZAP-70 enhances inhibition of ERK2, suggesting a role for VHR in modulating T-cell receptor responses (3). The enzymatic mechanism for VHR has been studied in detail (4) and its X-ray crystal structure has been characterized (5). Its well-defined biochemistry has made it useful in screening assays for compounds that inhibit phosphatases (6).

References

Ishibashi, T. et al. (1992) Proc. Natl. Acad. Sci. USA 89:12170.
Todd, J.L. et al. (1999) J. Biol. Chem. 274:13271.
Alonso, A. et al. (2003) Nat. Immunol. 4:44.
Zhang, Z.-Y. et al. (1995) Biochemistry 34:16088.
Yuvaniyama, J. et al. (1996) Science 272:1328.
Ueda, K. et al. (2002) FEBS Lett. 525:48.

Long Name

Vaccinia Virus VH1-related Phosphatase

Entrez Gene IDs

1845 (Human)

Alternate Names

dual specificity phosphatase 3; dual specificity protein phosphatase 3; Dual specificity protein phosphatase VHR; DUSP3; EC 3.1.3.16; EC 3.1.3.48; Vaccinia H1-related phosphatase; vaccinia virus phosphatase VH1-related; VHR; VHRserine/threonine specific protein phosphatase