Human/Mouse RelA/NFkB p65 PE-conjugated Antibody

Name: Human/Mouse RelA/NFkB p65 PE-conjugated Antibody
Brand: R&D Systems
SKU: IC5078P
Rating: 3 (1 reviews)

Catalog #: IC5078P
5 Citations 1 Review Datasheet / COA / SDS

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IC5078P

Detection of RelA/NF kappa B p65 in HeLa Human Cell Line by Flow Cytometry.

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All RelA/NFkB p65 Antibodies

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Citations (5)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse RelA/NFkB p65 PE-conjugated Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects human and mouse RelA/NF kappa B p65 in Western blots.

Source

Monoclonal Mouse IgG_2B Clone # 532301

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human RelA/NF kappa B p65 isoform 1
Asn456-Ser551
Accession # Q04206

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration

Sample

Intracellular Staining by Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry

Detection of RelA/NF?B p65 antibody in HeLa Human Cell Line antibody by Flow Cytometry.

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Detection of RelA/NF kappa B p65 in HeLa Human Cell Line by Flow Cytometry. HeLa human cervical epithelial carcinoma cell line was stained with Mouse Anti-Human/Mouse RelA/NF kappa B p65 PE-conjugated Monoclonal Antibody (Catalog # IC5078P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: RelA/NFkB p65

RelA p65 (v-rel reticuloendotheliosis viral oncogene homolog A) is a 65 kDa member of the NF kappa B family of nuclear transcription factors. Dimers of p65 with the p50 subunit are the most common form of the NF kappa B transcription factor, but dimers with it or other family members can also occur. Upon activation, RelA p65 forms an heterotetramer and moves into the nucleus where it binds to specific DNA sequences. An alternatively spliced isoform that lacks amino acids (aa) 222‑231 (p65 delta ) does not bind DNA. Over the sequence used as an immunogen, human RelA p65 shares 96% and 98% aa identity with mouse and rat RelA p65, respectively. This portion includes one of eight potential ser/thr phosphorylation sites, two acetylation sites, and most of the Rel homology domain that interacts with I kappa B inhibitors.

Long Name

v-rel Reticuloendotheliosis Viral Oncogene Homolog A

Entrez Gene IDs

5970 (Human)

Alternate Names

MGC131774; nf kb p65; NFkB p65; NF-kB p65; NFKB3; NFKB3v-rel avian reticuloendotheliosis viral oncogene homolog A (nuclear factor ofkappa light polypeptide gene enhancer in B-cells 3 (p65)); Nuclear factor NF-kappa-B p65 subunit; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3transcription factor p65; p65; p65RelA; rela p65; RelA; v-rel reticuloendotheliosis viral oncogene homolog A (avian); v-rel reticuloendotheliosis viral oncogene homolog A, nuclear factor of kappalight polypeptide gene enhancer in B-cells 3, p65

Product Datasheets

Product Datasheet

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Related Research Areas

Citations for Human/Mouse RelA/NFkB p65 PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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Autophagy is critical for group 2 innate lymphoid cell metabolic homeostasis and effector function
Authors: Galle-Treger L, Hurrell BP, Lewis G
J Allergy Clin Immunol.
DR3 stimulation of adipose resident ILC2s ameliorates type 2 diabetes mellitus
Authors: P Shafiei-Ja, BP Hurrell, L Galle-Treg, DG Helou, E Howard, J Painter, R Lo, G Lewis, P Soroosh, O Akbari
Nat Commun, 2020-09-18;11(1):4718.
CD200-CD200R immune checkpoint engagement regulates ILC2 effector function and ameliorates lung inflammation in asthma
Authors: P Shafiei-Ja, DG Helou, BP Hurrell, E Howard, C Quach, JD Painter, L Galle-Treg, M Li, YE Loh, O Akbari
Nature Communications, 2021-05-05;12(1):2526.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry
TNFR2 Signaling Enhances ILC2 Survival, Function, and Induction of Airway Hyperreactivity
Authors: BP Hurrell, L Galle-Treg, PS Jahani, E Howard, DG Helou, H Banie, P Soroosh, O Akbari
Cell Rep, 2019-12-24;29(13):4509-4524.e5.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry
Costimulation of type-2 innate lymphoid cells by GITR promotes effector function and ameliorates type 2 diabetes
Authors: L Galle-Treg, I Sankaranar, BP Hurrell, E Howard, R Lo, H Maazi, G Lewis, H Banie, AL Epstein, P Hu, VK Rehan, FD Gilliland, H Allayee, P Soroosh, AH Sharpe, O Akbari
Nat Commun, 2019-02-12;10(1):713.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry