Human/Mouse RIAM/APBB1IP Antibody

Catalog #: AF7279
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF7279
AF7279-SP

Detection of Human and Mouse RIAM/APBB1IP by Western Blot.

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All RIAM/APBB1IP Antibodies

Product Details

Citations (1)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse RIAM/APBB1IP Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects recombinant human RIAM/APBB1IP in direct ELISAs. Detects human and mouse RIAM/APBB1IP in Western blots

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human RIAM/APBB1IP
Gly2-Ser89
Accession # Q7Z5R6

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Immunocytochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human and Mouse RIAM/APBB1IP by Western Blot. Western blot shows lysates of THP-1 human acute monocytic leukemia cell line and EL-4 mouse lymphoblast cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human RIAM/APBB1IP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7279) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for RIAM/APBB1IP at approximately 100-110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Immunocytochemistry

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RIAM/APBB1IP in Wi-38 Human Cell Line. RIAM/APBB1IP was detected in immersion fixed Wi-38 human lung fibroblast cell line using Sheep Anti-Human RIAM/APBB1IP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7279) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper panel; Catalog # NL010) and counterstained with DAPI (blue, lower panel). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: RIAM/APBB1IP

APBB1IP (Amyloid beta A4 precursor protein-binding family B member 1 interacting protein; also PREL-1, Pro-rich protein 73, RARP1 and RIAM) is a member of the MRL family of proteins. Although its predicted MW is 73 kDa, it runs anomalously at 100-110 kDa in SDS-PAGE. It is expressed in megakaryocytes, T cells and fibroblasts. APBB1IP acts as a coordinator for Rap1 GTPase signaling. Rap1 both antagonizes Ras signaling, and is associated with beta 1 and beta 2 integrin-induced cell adhesion and cytoskeleton rearrangement. Within this context, APBB1IP operates as a cytosolic adaptor protein that induces integrin activation by recruiting talin and RAP1 to the cell membrane, and by mediating Profilin and VASP protein polymerizatiuon of actin. Human APBB1IP is 666 amino acids (aa) in length. It contains a poly-Pro sequence (aa 129-148), a Ras-association domain (aa 176-263), a PH domain (aa 310-419), and a large poly-Pro region (aa 503-640). There are multiple potential isoform variants. One contains a deletion of aa 152-177 coupled to a 27 aa substitution for aa 262-666, a second possesses a 21 aa substitution for aa 152‑666, and a third shows a deletion of aa 386-390 coupled to a five aa substitution for aa 417-666. Over aa 2-89, human APBB1IP shares 97% aa sequence identity with mouse APBB1IP.

Long Name

Amyloid Beta (A4) Precursor Protein-binding, Family B, Member 1 Interacting Protein

Entrez Gene IDs

54518 (Human); 54519 (Mouse); 307171 (Rat)

Alternate Names

amyloid beta (A4) precursor protein-binding, family B, member 1 interactingprotein; amyloid beta A4 precursor protein-binding family B member 1-interacting protein; APBB1-interacting protein 1; APBB1IP; INAG1; PREL1; PREL-1; proline rich EVH1 ligand 1; Proline-rich EVH1 ligand 1; Proline-rich protein 73; Rap1-GTP-interacting adapter molecule; Rap1-interacting adaptor molecule; RARP1; Retinoic acid-responsive proline-rich protein 1; RIAM; RIAMRARP-1

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Human/Mouse RIAM/APBB1IP Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1