Human/Mouse RUNX3/CBFA3 APC-conjugated Antibody

Catalog #: IC3765A
3 Citations Datasheet / COA / SDS

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IC3765A

Detection of RUNX3/CBFA3 in Human PBMC lymphocytes by Flow Cytometry.

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All RUNX3/CBFA3 Antibodies

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Citations (3)

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Human/Mouse RUNX3/CBFA3 APC-conjugated Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects human and mouse RUNX3/CBFA3 in Western blots.

Source

Monoclonal Mouse IgG_2A Clone # 527327

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human RUNX3/CBFA3
Lys186-Tyr415
Accession # Q13761

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration

Sample

Intracellular Staining by Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry

Detection of RUNX3/CBFA3 antibody in Human PBMC lymphocytes antibody by Flow Cytometry.

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Detection of RUNX3/CBFA3 in Human PBMC lymphocytes by Flow Cytometry. Human peripheral blood mononuclear cells (PBMC) lymphocytes either (A) untreated or (B) treated with PMA were stained with Mouse Anti-Human/Mouse RUNX3/CBFA3 APC-conjugated Monoclonal Antibody (Catalog # IC3765A, filled histogram) or isotype control antibody (Catalog # IC003A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: RUNX3/CBFA3

RUNX3, also known as CBFA3, AML-2 or PEBP2-alpha C, is a member of the Runt domain family of nuclear transcriptional regulators. All of the RUNX proteins form dimers with CBF-beta. The Runt domain, amino acids (aa) 54-186, is required for DNA binding, while a pro/ser/thr-rich region (aa 191-415) transcriptionally activates target genes. Isoform 2 has an alternate 19 aa in place of the N-terminal 5 aa of isoform 1. The 415 aa Human RUNX3 shares 91% aa identity with mouse or rat RUNX3. RUNX3 is necessary for growth control of gastric epithelium, neurogenesis of dorsal root ganglia, and T cell differentiation. RUNX3 expression is frequently mutated in tumors and appears to be silenced by methylation.

Long Name

Runt-related Transcription Factor 3

Entrez Gene IDs

864 (Human); 12399 (Mouse); 156726 (Rat)

Alternate Names

Acute myeloid leukemia 2 protein; AML2; AML2SL3/AKV core-binding factor alpha C subunit; CBFA3; CBFA3MGC16070; CBF-alpha-3; PEA2 alpha C; PEA2-alpha C; PEBP2 alpha C; PEBP2A3; PEBP2A3FLJ34510; PEBP2AC; PEBP2-alpha C; runt domain, alpha subunit 3; runt-related transcription factor 3; RUNX3; SL3-3 enhancer factor 1 alpha C subunit; transcription factor AML2

Product Datasheets

Product Datasheet

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Citations for Human/Mouse RUNX3/CBFA3 APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Single-cell CRISPR screens in vivo map T cell fate regulomes in cancer
Authors: Zhou, P;Shi, H;Huang, H;Sun, X;Yuan, S;Chapman, NM;Connelly, JP;Lim, SA;Saravia, J;Kc, A;Pruett-Miller, SM;Chi, H;
Nature
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry
Niche-specific MHC II and PD-L1 regulate CD4+CD8 alpha alpha + intraepithelial lymphocyte differentiation
Authors: Sookjin Moon, Yunji Park, Sumin Hyeon, Young-Min Kim, Ji-Hae Kim, Hyekang Kim et al.
Journal of Experimental Medicine
Langerhans cells are generated by two distinct PU.1-dependent transcriptional networks.
Authors: Chopin M, Seillet C, Chevrier S, Wu L, Wang H, Morse H, Belz G, Nutt S
J Exp Med, 2013-11-18;210(13):2967-80.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry