Human/Mouse S5a/Angiocidin Antibody

Catalog #: AF8036 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF8036
AF8036-SP

Detection of Human and Mouse S5a/Angiocidin by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse S5a/Angiocidin Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects human and mouse S5a/Angiocidin in Western blots.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human S5a/Angiocidin
Met1-Lys377
Accession # P55036

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human and Mouse S5a/Angiocidin by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, HeLa human cervical epithelial carcinoma cell line, AtT-20 mouse pituitary tumor cell line, IMR-32 human neuroblastoma cell line, and Neuro-2A mouse neuroblastoma cell line. PVDF membrane was probed with 2 µg/mL of Sheep Anti-Human/Mouse S5a/Angiocidin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8036) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for S5a/Angiocidin at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: S5a/Angiocidin

S5a (26S proteasome regulatory subunit S5a/RPN10/nonATPase 4; also Angiocidin, ASF and multiubiquitin chain-binding protein) is a 50-60 kDa protein that belongs to the proteasome subunit S5A family of molecules. It is ubiquitously expressed, and found in both cytosol and in association with the 26S proteosome. Intracellularly, proteins targeted for degradation are marked by ubiquitin (Ub), a 9 kDa polypeptide. Following ubiquitination, proteins so marked are recognized by a degradation unit composed of three complexes, a central 20S complex and two flanking 19S complexes. The 20S barrel-shaped complex contains 28 subunits, one of which is S5a. This subunit binds Lys48 multiubiquitinated proteins, and contributes to proteosome-based proteolysis. S5a also binds proteins with intrinsic Ub-like sequences, and is posited to contribute to their degradation. Notably, S5a interacts with E3/E2 pairs to insure that non-forked Ub chains appear on target proteins. Failure to do so leads to forked Ub chains that are poor proteosome targets. S5a also exists in the circulation, and appears to bind TSP-1. Extracellularly, S5a is reported to possess both antiangiogenic and antiproliferative activity, and to promote inflammatory mediator secretion from mononuclear cells. Human S5a is synthesized as a 377 amino acid (aa) protein that contains one vWF-A type domain (aa 5-188) and two UIM, or Ub-interacting motifs (aa 211-230 and 282-301). There are at least three utilized Ser phosphorylation sites. Although mouse S5a has four reported splice forms, human appears to have but two, one that contains a GlyGluArg insertion after Glu254 (called angiocidin), and a second 32-33 kDa isoform that exists in the embryo and contains a 14 aa substitution for aa 255-377. Full-length human S5a shares 96% aa sequence identity with mouse S5a.

Long Name

26S Proteasome Regulatory Subunit S5a

Entrez Gene IDs

5710 (Human); 19185 (Mouse); 83499 (Rat)

Alternate Names

26S proteasome non-ATPase regulatory subunit 4;AF;AF-1;ASF;MCB1;PSMD4;pUB-R5;Rpn10;S5A; Angiocidin; ASF; Macropain; Mcb1; PSMD4; pUB-R5; Rpn10 ; S5a