Human/Mouse SCAMP3 Antibody

Name: Human/Mouse SCAMP3 Antibody
Brand: R&D Systems
SKU: AF5344
Rating: 5 (1 reviews)

Catalog #: AF5344 1 Review Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF5344
AF5344-SP

Detection of Human/Mouse SCAMP3 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse SCAMP3 Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects endogenous human and mouse SCAMP3 in Western blots.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human SCAMP3
Met1-Leu170
Accession # O14828

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human/Mouse SCAMP3 by Western Blot. Western blot shows lysates of NIH-3T3 mouse embryonic fibroblast cell line and K562 human chronic myelogenous leukemia cell line, HepG2 human hepatocellular carcinoma cell line, and JEG-3 human epithelial choriocarcinoma cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse SCAMP3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5344) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for SCAMP3 at approximately 38 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SCAMP3

SCAMP3 (Secretory carrier membrane protein 3) is a 38‑40 kDa member of the SCAMP family of proteins. It is expressed in heart and skeletal muscle, plus fat, and functions in post-Golgi recycling and EGFR internalization pathways. Human SCAMP3 is 347 amino acids (aa) in length. It contains a cytoplasmic N-terminus (aa 1‑170) that shows three NPF repeats (binding sites for EH domain proteins), a heptad repeat of aliphatic aa's, and a potential SH3-binding motif. This is followed by four consecutive transmembrane domains (aa 171‑297) plus a C-terminal cytoplasmic region (aa 298‑347). It appears that phosphorylation of SCAMP3 on Tyr41 is a requisite for its interaction with EGFR. There are three potential splice forms. One shows a deletion of aa 23‑48, while two others show a one and 23 aa substitution for aa 131‑144 and 131‑260, respectively. Over aa 1‑170, human and mouse SCAMP3 are 91% aa identical.

Long Name

Secretory Carrier Membrane Protein 3

Entrez Gene IDs

10067 (Human); 24045 (Mouse); 65169 (Rat)

Alternate Names

propin 1; PROPIN1; SCAMP3; secretory carrier membrane protein 3C1orf3; secretory carrier-associated membrane protein 3