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Human/Mouse Semaphorin 3E Antibody

Catalog #: AF3239
9 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF3239
AF3239-SP
Semaphorin 3E in Mouse Brain.
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Citations (9)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse Semaphorin 3E Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human Semaphorin 3E in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant human (rh) Semaphorin 3A, rhSemaphorin 3C, rhSemaphorin 3F, and rhSemaphorin 3G is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Semaphorin 3E
Thr25-Ser775 (Arg557Ala and Arg560Ala)
Accession # O15041
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human Semaphorin 3E (Catalog # 3239-S3)
Immunohistochemistry
5-15 µg/mL
See below
Immunocytochemistry
5-15 µg/mL
Immersion fixed MDA-MB-453 human breast cancer cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry Semaphorin 3E antibody in Mouse Brain by Immunohistochemistry (IHC-Fr). View Larger

Semaphorin 3E in Mouse Brain. Semaphorin 3E was detected in immersion fixed frozen sections of adult mouse brain using Goat Anti-Human Semaphorin 3E Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3239) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Northern-Lights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cerebellum. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Semaphorin 3E

Semaphorin 3E (Sema3E; previously SemaH) is one of six Class 3 (secreted) semaphorins which in the human share 40-50% amino acid (aa) identity. Class 3 semaphorins are potent chemorepellents that function in axon guidance and/or vascular tip cell guidance during development (1). Sema3E is highly expressed by a subset of motor neurons in developing somites, where it acts as a repulsive cue for PlexinD1-expressing endothelial cells of adjacent intersomitic vessels (2, 3). Crystal structures of semaphorins reveal that the 500 aa N-terminal Sema domain forms a seven-blade b-propeller similar to that found in integrin molecules; 14 conserved cysteine residues and one or more N-glycosylation sites are thought critical for forming the secondary structure (4). C-terminal to the Sema domain, Sema3E has a consensus sequence for furin cleavage which, when used, creates a 61kDa form that does not dimerize and is highly expressed in tumor cell lines with metastatic potential (5, 6). Further C-terminal are a cysteine-knot plexin/semaphorin/integrin (PSI) domain, an Ig-like domain, a cysteine for dimerization and a basic domain containing another furin site. Dimerization and cleavage at the C-terminal site are required for repulsing activity of class 3 semaphorins (7). Human Sema3E shares 90%, 85% and 57% aa identity with mouse, cow and dog Sema3E, respectively. Like other semaphorins, Sema3E signaling is transduced by a transmembrane Plexin dimer, which also has a Sema domain and is coupled to kinase pathways. Unlike other Class 3 semaphorins, Sema3E binds directly to its plexin and does not require interaction with a neuropilin for activity (7). Genetic disruption of either Sema3E or PlexinD1 creates mouse mutants with excessive and disorganized vascular growth and branching, indicating the importance of this ligand-receptor pair for vascular guidance (3, 8).

References
  1. Eichmann, A. et al. (2005) Genes Dev. 19:1013.
  2. Cohen, S. et al. (2005) Eur. J. Neurosci. 21:1767.
  3. Gu, C. et al. (2005) Science 307:265.
  4. Gherardi, E. et al. (2004) Curr. Opin. Struct. Biol. 14:669.
  5. Christensen, C. et al. (1998) Cancer Res. 58:1238.
  6. Christensen, C. et al. (2005) Cancer Res. 65:6167.
  7. Adams, R. H. et al. (1997) EMBO J. 16:6077.
  8. Gitler, A. D. et al. (2004) Developmental Cell 7:107.
Entrez Gene IDs
9723 (Human); 20349 (Mouse)
Alternate Names
(semaphorin) 3E; coll-5; KIAA0331M-SEMAH; sema domain, immunoglobulin domain (Ig), short basic domain, secreted; Sema3E; SEMAH; SEMAHM-SemaK; Semaphorin 3E; semaphorin-3E

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Citations for Human/Mouse Semaphorin 3E Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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  1. The Expression of Semaphorin3E in Vagal Ganglion and Lung Tissue Is Related to Airway Hyperresponsiveness in Murine Asthma Model
    Authors: Chen, L;Yuan, X;He, Y;Fan, Z;Guan, Y;Li, Q;Chen, Y;Bao, L;Huang, Y;Lai, K;
    Journal of immunology research
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Immunohistochemistry
  2. Plexin D1 mediates disturbed flow-induced M1 macrophage polarization in atherosclerosis
    Authors: Zhang S, Zhang Y, Zhang P et al.
    Heliyon
  3. A Novel Loss-of-Function SEMA3E Mutation in a Patient with Severe Intellectual Disability and Cognitive Regression
    Authors: AJJ Paganoni, F Amoruso, J Porta Pela, B Calleja-Pé, V Vezzoli, P Duminuco, A Caramello, R Oleari, A Fernández-, A Cariboni
    International Journal of Molecular Sciences, 2022-05-18;23(10):.
    Species: Mouse, Primate - C. aethiops
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. Mesenchymal Stromal Cells Promote Retinal Vascular Repair by Modulating Sema3E and IL-17A in a Model of Ischemic Retinopathy
    Authors: Baraa Noueihed, José Carlos Rivera, Rabah Dabouz, Pénélope Abram, Samy Omri, Isabelle Lahaie et al.
    Frontiers in Cell and Developmental Biology
  5. TG-interacting factor 1 (Tgif1)-deficiency attenuates bone remodeling and blunts the anabolic response to parathyroid hormone
    Authors: H Saito, A Gasser, S Bolamperti, M Maeda, L Matthies, K Jähn, CL Long, H Schlüter, M Kwiatkowsk, V Saini, PD Pajevic, T Bellido, AJ van Wijnen, KS Mohammad, TA Guise, H Taipaleenm, E Hesse
    Nat Commun, 2019-03-22;10(1):1354.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  6. Expression of Semaphorin 3E Is Suppressed in Severe Asthma
    Authors: H Movassagh, L Shan, J Chakir, JF McConville, AJ Halayko, L Koussih, AS Gounni
    J. Allergy Clin. Immunol., 2017-05-12;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  7. Dysfunctional SEMA3E signaling underlies gonadotropin-releasing hormone neuron deficiency in Kallmann syndrome
    Authors: Anna Cariboni, Valentina André, Sophie Chauvet, Daniele Cassatella, Kathryn Davidson, Alessia Caramello et al.
    Journal of Clinical Investigation
  8. Neuroimmune guidance cue Semaphorin 3E is expressed in atherosclerotic plaques and regulates macrophage retention.
    Authors: Wanschel, Amarylis, Seibert, Tara, Hewing, Bernd, Ramkhelawon, Bhama, Ray, Tathagat, van Gils, Janine M, Rayner, Katey J, Feig, Jonathan, O'Brien, Edward R, Fisher, Edward A, Moore, Kathryn
    Arterioscler Thromb Vasc Biol, 2013-02-21;33(5):886-93.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  9. Dynamics of the skeletal muscle secretome during myoblast differentiation.
    Authors: Henningsen J, Rigbolt K, Blagoev B, Pedersen B, Kratchmarova I
    Mol Cell Proteomics, 2010-07-14;9(11):2482-96.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot

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