Human/Mouse sFRP-3 Antibody Summary
Ala33-Asn325
Accession # AAB51298
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human sFRP‑3 by Western Blot. Western blot shows lysates of SH-SY5Y human neuroblastoma cell line and PANC-1 human pancreatic carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse sFRP-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF192) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for sFRP-3 at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: sFRP-3
Secreted Frizzled Related Protein 3 (sFRP-3) was originally identified in bovine cartilage for its chondrogenic ability. Human, mouse, chick and Xenopus clones have also been isolated. SFRP-3 is often referred to as FRZB, other names include Fritz, Frzb1, and FRP-3. At the amino acid sequence level, sFRP-3 is highly conserved. The human protein shares 77% identity with Xenopus, 92% with mouse, and 94% with bovine proteins. Human sFRP-3 is strongly expressed in the developing appendicular skeleton, and cartilage of craniofacial bones. As determined by Northern blot of adult tissues, it is strongly detected in heart and placenta, as well as the brain, skeletal muscle, kidney and pancreas.
The N-terminal portion of the human protein shows 50% amino acid identity to the corresponding region of the Drosophila frizzled gene product, a receptor for Wg/Wnt signals. The similarity of sFRP-3 with frizzled proteins is restricted to the N-terminal cysteine-rich domain (CRD) that contains at least ten cysteine residues with highly conserved spacing between them. SFRP-3 was subsequently shown to be a soluble antagonist of Wnt signals. It lacks all transmembrane domains of frizzled proteins but retains the ability to bind Wnts. Ectopic expression of sFRP-3 mRNA has been shown to interfere with the induction of secondary axes in Xenopus embryos injected with Xwnt-8 mRNA.
- Hoang, et al. (1996) J. Biol. Chem. 271:26131.
- Leyns, et al. (1997) Cell 88:747.
- Wang, et al. (1997) Cell 88:757.
- Mayr, et al. (1997) Mech. Dev. 63:109.
- Rattner, et al. (1997) PNAS 94:2859.
Product Datasheets
Citations for Human/Mouse sFRP-3 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 4
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Expression of SFRP Family Proteins in Human Keratoconus Corneas
Authors: Jingjing You, Li Wen, Athena Roufas, Michele C. Madigan, Gerard Sutton
PLoS ONE
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Frizzled-related protein variants are risk factors for hip osteoarthritis.
Authors: Lane NE, Lian K, Nevitt MC, Zmuda JM, Lui L, Li J, Wang J, Fontecha M, Umblas N, Rosenbach M, de Leon P, Corr M
Arthritis Rheum., 2006-04-01;54(4):1246-54.
Species: Human
Sample Types: Serum
Applications: ELISA Development -
Functional variants within the secreted frizzled-related protein 3 gene are associated with hip osteoarthritis in females.
Authors: Loughlin J, Dowling B, Chapman K, Marcelline L, Mustafa Z, Southam L, Ferreira A, Ciesielski C, Carson DA, Corr M
Proc. Natl. Acad. Sci. U.S.A., 2004-06-21;101(26):9757-62.
Species: Human
Sample Types: Cell Lysates, Whole Tissue
Applications: IHC-Fr, Western Blot -
Short-term retinoic acid treatment sustains pluripotency and suppresses differentiation of human induced pluripotent stem cells
Authors: MT De Angelis, EI Parrotta, G Santamaria, G Cuda
Cell Death Dis, 2018-01-05;9(1):6.
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