Human/Mouse SOCS-3 Antibody Summary
Met1-Leu225
Accession # O14543
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human and Mouse SOCS‑3 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, Jurkat human acute T cell leukemia cell line, U937 human histiocytic lymphoma cell line, and C2C12 mouse myoblast cell line. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human/Mouse SOCS-3 Monoclonal Antibody (Catalog # MAB5696) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for SOCS-3 at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: SOCS-3
Suppressor of cytokine signaling 3 (SOCS-3), also known as CIS3, is a widely expressed 30 kDa protein that inhibits signal transduction induced by multiple cytokines including Erythropoietin, IL-6, LIF, Oncostatin M, and Leptin. It binds downstream signaling molecules such as JAK kinases and targets them for ubiquitin-mediated degradation, essential in a negative feedback control loop. Human SOCS-3 shares 96% aa sequence identity with mouse and rat SOCS-3.
Product Datasheets
Citations for Human/Mouse SOCS-3 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 6
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ML218 HCl Is More Efficient Than Capsaicin in Inhibiting Bacterial Antigen-Induced Cal 27 Oral Cancer Cell Proliferation
Authors: R Chakrabort, H Hu, C Darido, K Vickery, S Ranganatha
International Journal of Molecular Sciences, 2021-11-22;22(22):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Bacterial Antigens Reduced the Inhibition Effect of Capsaicin on Cal 27 Oral Cancer Cell Proliferation
Authors: R Chakrabort, K Vickery, C Darido, S Ranganatha, H Hu
International Journal of Molecular Sciences, 2021-08-12;22(16):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
An activity-dependent determinant of synapse elimination in the mammalian brain
Authors: Masahiro Yasuda, Sivapratha Nagappan-Chettiar, Erin M. Johnson-Venkatesh, Hisashi Umemori
Neuron
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IL-24 Promotes Pseudomonas aeruginosa Keratitis in C57BL/6 Mouse Corneas
Authors: BX Ross, N Gao, X Cui, TJ Standiford, J Xu, FX Yu
J. Immunol, 2017-03-22;0(0):.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Expression of suppressor of cytokine signaling genes in human elderly and Alzheimer’s disease brains and human microglia
Authors: D.G. Walker, A.M. Whetzel, L.-F. Lue
Neuroscience
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Dysregulation of the suppressor of cytokine signalling 3-signal transducer and activator of transcription-3 pathway in the aetiopathogenesis of Sjogren's syndrome.
Authors: Vartoukian S, Tilakaratne W, Seoudi N, Bombardieri M, Bergmeier L, Tappuni A, Fortune F
Clin Exp Immunol, 2014-09-01;177(3):618-29.
Species: Human
Sample Types: Cell Lysates, Whole Tissue
Applications: IHC-P, Western Blot
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