Human/Mouse SOD1/Cu-Zn SOD Antibody

Name: Human/Mouse SOD1/Cu-Zn SOD Antibody
Brand: R&D Systems
SKU: AF3418
Rating: 4 (1 reviews)

Catalog #: AF3418
6 Citations 1 Review Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF3418
AF3418-SP

Detection of Human/Mouse SOD1/Cu‑Zn SOD by Western Blot.

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All SOD1/Cu-Zn SOD Antibodies

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Citations (6)

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Reviews (1)

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse SOD1/Cu-Zn SOD Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects human and mouse SOD1/Cu‑Zn SOD in Western blots. Detects rat SOD1, but Catalog # AF3787 is recommended.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human SOD1/Cu‑Zn SOD
Met1-Gln154
Accession # P00441

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.2 µg/mL

See below

Simple Western

10 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human/Mouse SOD1/Cu‑Zn SOD by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 0.2 µg/mL Goat Anti-Human/Mouse SOD1/Cu-Zn SOD Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3418) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). For additional reference, recombinant human SOD1, SOD2, and SOD3 (1 ng/lane) were included. A specific band for SOD1/Cu-Zn SOD was detected at approximately 16-19 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Simple Western

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Detection of Human SOD1/Cu‑Zn SOD by Simple Western^TM. Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for SOD1/Cu-Zn SOD at approximately 25 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse SOD1/Cu-Zn SOD Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3418) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Simple Western

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Detection of Human SOD1/Cu‑Zn SOD by Simple Western^TM. Simple Western lane view shows lysates of A549 human lung carcinoma cell line and SK-BR-3 human breast cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for SOD1/Cu-Zn SOD at approximately 24 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse SOD1/Cu-Zn SOD Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3418) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SOD1/Cu-Zn SOD

Superoxide Dismutases (SODs), originally identified as Indophenoloxidase (IPO), are enzymes that catalyze the converversion of naturally-occuring but harmful superoxide radicals into molecular oxygen and hydrogen peroxide. Superoxide Dismutases 1, SOD1, also known as Cu/Zn SOD, soluble SOD, and IPO-A, is a soluble, cytoplasmic 16 kDa homodimer. Each SOD1 monomer binds one Cu²⁺ and Zn²⁺ ion. Three isozymes of SOD have been identified and are functionally related but have very modest sequence homology. SOD1 shares 23% and 27% sequence identity with SOD2 and SOD3, respectively. Mutations in SOD1 have been suggested to be the cause of familial amyotrophic lateral sclerosis (ALS). The ALS-causing mutations of SOD1 are scattered throughout the protein and provide no clear functional or structural clues to the underlying disease mechanism. The oligomerization hypothesis suggests that mutant SOD1 proteins become misfolded and consequently oligomerize into high molecular weight aggregates that result in the death of motor neurons. The oxidative damage hypothesis suggests that loss of function mutations in SOD1 result in the intracellular accumulation of the superoxide radical, leading to free radical-mediated damage, the release of cytochrome c, and apoptosis.

Long Name

Superoxide Dismutase-1

Entrez Gene IDs

6647 (Human); 20655 (Mouse); 24786 (Rat)

Alternate Names

ALS; ALS1; amyotrophic lateral sclerosis 1 (adult); Cu; Cu/Zn superoxide dismutase; CuZn SOD; Cu-Zn SOD; EC 1.15.1.1; homodimer; hSod1; indophenoloxidase A; Ipo1; IPOA; SOD; SOD, cytosolic; SOD, Soluble; SOD1; superoxide dismutase [Cu-Zn]; Superoxide dismutase 1; superoxide dismutase 1, soluble; Zn superoxide dismutase, EC 1.15.1.110superoxide dismutase, cystolic

Product Datasheets

Product Datasheet

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Related Research Areas

Citations for Human/Mouse SOD1/Cu-Zn SOD Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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A diacidic motif determines unconventional secretion of wild-type and ALS-linked mutant SOD1
Authors: David Cruz-Garcia, Nathalie Brouwers, Juan M. Duran, Gabriel Mora, Amy J. Curwin, Vivek Malhotra
Journal of Cell Biology
Chronic Restraint Stress Induces Gastric Mucosal Inflammation with Enhanced Oxidative Stress in a Murine Model

Authors: Maimaiti Yisireyili, Aziguli Alimujiang, Aikebaier Aili, Yiliang Li, Salamaiti Yisireyili, Kelimu Abudureyimu
Psychology Research and Behavior Management
MSC Pretreatment for Improved Transplantation Viability Results in Improved Ventricular Function in Infarcted Hearts
Authors: MF Pittenger, S Eghtesad, PG Sanchez, X Liu, Z Wu, L Chen, BP Griffith
International Journal of Molecular Sciences, 2022-01-08;23(2):.
Species: Rat
Sample Types: Cell Lysates
Applications: Western Blot
CETSA-based target engagement of taxanes as biomarkers for efficacy and resistance
Authors: A Langebäck, S Bacanu, H Laursen, L Mout, T Seki, S Erkens-Sch, AD Ramos, A Berggren, Y Cao, J Hartman, W van Weerde, J Bergh, P Nordlund, S Lööf
Sci Rep, 2019-12-18;9(1):19384.
Species: Human, Xenograft
Sample Types: Beads
Applications: Immunoassay
Neuroprotective and neuritogenic activities of novel multimodal iron-chelating drugs in motor-neuron-like NSC-34 cells and transgenic mouse model of amyotrophic lateral sclerosis.
Authors: Kupershmidt L, Weinreb O, Amit T, Mandel S, Carri MT, Youdim MB
FASEB J., 2009-07-28;23(11):3766-79.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
Rejuvenation of mesenchymal stem cells by extracellular vesicles inhibits the elevation of reactive oxygen species
Authors: Vuong Cat Khanh, Toshiharu Yamashita, Kinuko Ohneda, Chiho Tokunaga, Hideyuki Kato, Motoo Osaka et al.
Scientific Reports