Human/Mouse Somatostatin Antibody Summary
Accession # P61278
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Somatostatin in Human Embryonic Stem Cells. Somatostatin was detected in immersion fixed human embryonic stem cells differentiated to pancreatic cells using Rat Anti-Human Somatostatin Monoclonal Antibody (Catalog # MAB2358) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; NL013) and counterstained with DAPI (blue). Specific staining was localized to cell membranes and cytoplasm. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.
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Somatostatin in beta TC‑6 Mouse Cell Line. Somatostatin was detected in immersion fixed beta TC-6 mouse beta cell insulinoma cell line using Rat Anti-Human Somatostatin Monoclonal Antibody (Catalog # MAB2358) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; NL013) and counterstained with DAPI (blue). Specific staining was localized to cell membranes and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
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Somatostatin in Human Pancreas. Somatostatin was detected in immersion fixed paraffin-embedded sections of human pancreas using Rat Anti-Human/Mouse Somatostatin Monoclonal Antibody (Catalog # MAB2358) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to islet cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
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Detection of Somatostatin in TT human medullary thyroid cancer cell line (Positive) & K562 human chronic myelogenous leukemia cell line (Negative). Somatostatin was detected in immersion fixed TT human medullary thyroid cancer cell line (Positive) & K562 human chronic myelogenous leukemia cell line (Negative) using Rat Anti-Human/Mouse Somatostatin Monoclonal Antibody (Catalog # MAB2358) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Somatostatin
Somatostatin (SST), also known as SMST, is a 116 amino acid (aa) peptide hormone protein and shares 96% aa identity with mouse and rat SST. It exists as a preproprotein that is cleaved into a proprotein form and then subsequently into 14 aa and 28 aa active peptide forms. SST is secreted by many tissues throughout the body and inhibits the secretion of many other hormones. Some of its major roles are inhibiting growth hormone secretion by the pituitary gland, insulin and glucagon secretion in pancreatic islets, and secretion of gastrointestinal tract hormones such as gastrin. Additionally, SST has been shown to have neuromodulatory activity.
Product Datasheets
Citations for Human/Mouse Somatostatin Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 3
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SARS-CoV-2 infection induces beta cell transdifferentiation
Authors: Tang X, Uhl S, Zhang T et al.
Cell Metabolism
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An Autaptic Culture System for Standardized Analyses of iPSC-Derived Human Neurons
Authors: HJ Rhee, AH Shaib, K Rehbach, C Lee, P Seif, C Thomas, E Gideons, A Guenther, T Krutenko, M Hebisch, M Peitz, N Brose, O Brüstle, JS Rhee
Cell Rep, 2019-05-14;27(7):2212-2228.e7.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Enteroendocrine cell lineages that differentially control feeding and gut motility
Authors: Marito Hayashi, Judith A Kaye, Ella R Douglas, Narendra R Joshi, Fiona M Gribble, Frank Reimann et al.
eLife
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Somatostatin is shown in green. Parvalbumin is shown in purple. NeuN is shown in white.
