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Human/Mouse Sonic Hedgehog/Shh N‑Terminus Antibody

Catalog #: AF464
33 Citations 2 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF464
AF464-SP
Detection of Human and Mouse Sonic Hedgehog/Shh by Western Blot.
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Product Details
Citations (33)
FAQs
Supplemental Products
Reviews (2)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse Sonic Hedgehog/Shh N‑Terminus Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human and mouse Sonic Hedgehog/Shh N‑Terminus in direct ELISAs and Western blots. In direct ELISAs, less than
15% cross‑reactivity with recombinant mouse (rm) Dhh N‑Terminus and rmIhh N-Terminus is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse Sonic Hedgehog/Shh N‑Terminus
Cys25-Gly198 (Lys122Arg)
Accession # Q62226
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human and Mouse Sonic Hedgehog/Shh antibody by Western Blot. View Larger

Detection of Human and Mouse Sonic Hedgehog/Shh by Western Blot. Western blot shows lysates of human stomach tissue and mouse stomach tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse Sonic Hedgehog/Shh N-Terminus Antigen Affinity-purified Polyclonal Antibody (Catalog # AF464) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Sonic Hedgehog/Shh at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry Sonic Hedgehog/Shh antibody in Mouse Embryo by Immunohistochemistry (IHC-Fr). View Larger

Sonic Hedgehog/Shh in Mouse Embryo. Sonic Hedgehog/Shh was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c.) using Goat Anti-Human/Mouse Sonic Hedgehog/Shh N-Terminus Antigen Affinity-purified Polyclonal Antibody (Catalog # AF464) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to developing brain. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Sonic Hedgehog/Shh

The hedgehog (hh) gene encoding a secreted protein was originally identified in Drosophila as a segment polarity gene. The vertebrate homologues of Hh comprise several proteins including sonic hedgehog (Shh), Indian hedgehog (Ihh), and Desert hedgehog (Dhh). Hedgehog proteins are important signaling molecules during embryonic development. Shh genes are highly conserved and have been identified in a variety of species including human, mouse, frog, fish, and chicken. Mouse and human Shh are 92% identical at the amino acid sequence level. Shh is expressed in key embryonic tissues such as the Hensen’s node, the zone of polarizing activity in the posterior limb bud, the notochord, and the floor plate of the neural tube. Shh is involved in regulating the patterning of the developing central nervous system, somite, and limb. Shh plays an important role in the development of particular tissues such as whisker, hair, foregut, tooth and bone. Evidence also suggests that Shh is involved in regulating stem cell fates of neural and hematopoeitic lineages, and that aberrant Shh signaling is implicated in basal cell carcinomas and other diseases.

Mouse Shh cDNA encodes a 437 amino acid residue with a predicted 24 aa residue signal peptide that is cleaved to generate a 413 aa residue precursor protein. An autocatalytic reaction yields a 19 kDa amino-terminal domain Shh-N protein containing cholesterol and palmitate, and a 27 kDa carboxy-terminal domain Shh-C protein. The N-terminal domain retains all known signaling capabilities, while the C-terminal domain is responsible for the intramolecular processing, acting as a cholesterol transferase. Shh can act as both a short-range contact dependent factor and as a long-range, diffusible morphogen. At the cell surface, Shh activity is mediated by a multicomponent receptor complex involving the 12-pass transmembrane protein Patched (Ptc) which binds Shh with high affinity and Smoothened (Smo), a signaling seven transmembrane G-protein coupled receptor. In the absence of Shh, Ptc represses Smo activity. The binding of Shh to Ptc, releases the basal repression of Smo by Ptc (1‑5).

References
  1. Carpenter, D. et al. (1998) Proc. Natl. Acad. Sci. USA 95:13630.
  2. Perrimon, N. (1995) Cell 80:517.
  3. Weed, M. et al. (1997) Matrix Biol. 16:53.
  4. Mullor, J. et al. (2002) Trends Cell Biol. 12:562.
  5. Ingham, P. and A. McMahon (2001) Genes & Dev. 15:3059.
Entrez Gene IDs
6469 (Human); 20423 (Mouse)
Alternate Names
HHG1; HHG-1; HLP3; HPE3; MCOPCB5; MCOPCB5sonic hedgehog (Drosophila) homolog; Shh; ShhNC; SMMCI; SMMCIsonic hedgehog homolog (Drosophila); sonic hedgehog homolog; sonic hedgehog protein; Sonic Hedgehog; TPT; TPTPS

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Citations for Human/Mouse Sonic Hedgehog/Shh N‑Terminus Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

33 Citations: Showing 1 - 10
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  1. Disrupting Hedgehog Cardin–Weintraub sequence and positioning changes cellular differentiation and compartmentalization in vivo
    Authors: Philipp Kastl, Dominique Manikowski, Georg Steffes, Sabine Schürmann, Shyam Bandari, Christian Klämbt et al.
    Development
  2. Timing of Smarcb1 and Nf2 inactivation determines schwannoma versus rhabdoid tumor development
    Authors: Vitte J, Gao F, Coppola G et al.
    Nat Commun
  3. Multiple Shh signaling centers participate in fungiform papilla and taste bud formation and maintenance.
    Authors: Liu HX, Ermilov A, Grachtchouk M et al.
    Dev Biol
  4. Unique lingual expression of the Hedgehog pathway antagonist Hedgehog-interacting protein in filiform papillae during homeostasis and ectopic expression in fungiform papillae during Hedgehog signaling inhibition
    Authors: Kumari A, Li L, Ermilov AN et al.
    Developmental dynamics : an official publication of the American Association of Anatomists
  5. Early taste buds are from Shh+ epithelial cells of tongue primordium in distinction from mature taste bud cells which arise from surrounding tissue compartments
    Authors: Naomi Kramer, Guiqian Chen, Mohamed Ishan, Xiaogang Cui, Hong-Xiang Liu
    Biochemical and Biophysical Research Communications
  6. Proteolytic processing of palmitoylated Hedgehog peptides specifies the 3-4 intervein region of the Drosophila wing
    Authors: Sabine Schürmann, Georg Steffes, Dominique Manikowski, Philipp Kastl, Ursula Malkus, Shyam Bandari et al.
    eLife
  7. Conserved cholesterol-related activities of Dispatched 1 drive Sonic hedgehog shedding from the cell membrane
    Authors: Kristina Ehring, Dominique Manikowski, Jonas Goretzko, Jurij Froese, Fabian Gude, Petra Jakobs et al.
    Journal of Cell Science
  8. Taste papilla cell differentiation requires tongue mesenchyme via ALK3-BMP signaling to regulate the production of secretory proteins
    Authors: M Ishan, Z Wang, P Zhao, Y Yao, S Stice, L Wells, Y Mishina, HX Liu
    bioRxiv : the preprint server for biology, 2023-04-04;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  9. Probing the multimodal fungiform papilla: complex peripheral nerve endings of chorda tympani taste and mechanosensitive fibers before and after Hedgehog pathway inhibition
    Authors: Donnelly CR, Kumari A, Li L et al.
    Cell and Tissue Research
  10. Wnt Inhibitory Factor 1 Binds to and Inhibits the Activity of Sonic Hedgehog
    Authors: K Kerekes, M Trexler, L Bányai, L Patthy
    Cells, 2021-12-10;10(12):.
    Species: Human
    Sample Types: Protein
    Applications: Western Blot
  11. Sonic hedgehog signalling as a potential endobronchial biomarker in COPD
    Authors: J Ancel, R Belgacemi, JM Perotin, Z Diabasana, S Dury, M Dewolf, A Bonnomet, N Lalun, P Birembaut, M Polette, G Deslée, V Dormoy
    Respir. Res., 2020-08-07;21(1):207.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  12. Airway epithelial cell differentiation relies on deficient Hedgehog signalling in COPD
    Authors: R Belgacemi, E Luczka, J Ancel, Z Diabasana, JM Perotin, A Germain, N Lalun, P Birembaut, X Dubernard, JC Mérol, G Delepine, M Polette, G Deslée, V Dormoy
    EBioMedicine, 2019-12-23;51(0):102572.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  13. Functionally Distinctive Ptch Receptors Establish Multimodal Hedgehog Signaling in the Tooth Epithelial Stem Cell Niche
    Authors: M Binder, P Chmielarz, PJ Mckinnon, LC Biggs, I Thesleff, A Balic
    Stem Cells, 2019-06-10;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  14. Species generalization and differences in Hedgehog pathway regulation of fungiform and circumvallate papilla taste function and somatosensation demonstrated with sonidegib
    Authors: A Kumari, Y Yokota, L Li, RM Bradley, CM Mistretta
    Sci Rep, 2018-11-01;8(1):16150.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  15. Maintenance of Taste Organs Is Strictly Dependent on Epithelial Hedgehog/GLI Signaling
    PLoS Genet, 2016-11-28;12(11):e1006442.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  16. Ectoderm mesenchymal stem cells promote differentiation and maturation of oligodendrocyte precursor cells
    Authors: Zhijian Zhang
    Biochem. Biophys. Res. Commun, 2016-10-28;0(0):.
    Species: Rat
    Sample Types: Cell Culture Supernates, Whole Cells
    Applications: ICC, Western Blot
  17. Smoothened Agonist Reduces Human Immunodeficiency Virus Type-1-Induced Blood-Brain Barrier Breakdown in Humanized Mice
    Authors: Vir B Singh
    Sci Rep, 2016-05-31;6(0):26876.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  18. Transcription factor 7-like 1 is involved in hypothalamo-pituitary axis development in mice and humans.
    Authors: Gaston-Massuet C, McCabe M, Scagliotti V, Young R, Carreno G, Gregory L, Jayakody S, Pozzi S, Gualtieri A, Basu B, Koniordou M, Wu C, Bancalari R, Rahikkala E, Veijola R, Lopponen T, Graziola F, Turton J, Signore M, Mousavy Gharavy S, Charolidi N, Sokol S, Andoniadou C, Wilson S, Merrill B, Dattani M, Martinez-Barbera J
    Proc Natl Acad Sci U S A, 2016-01-13;113(5):E548-57.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  19. Prospectively isolated NGN3-expressing progenitors from human embryonic stem cells give rise to pancreatic endocrine cells.
    Authors: Cai, Qing, Bonfanti, Paola, Sambathkumar, Rangaraj, Vanuytsel, Kim, Vanhove, Jolien, Gysemans, Conny, Debiec-Rychter, Maria, Raitano, Susanna, Heimberg, Harry, Ordovas, Laura, Verfaillie, Catherin
    Stem Cells Transl Med, 2014-02-03;3(4):489-99.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  20. Loss of the transcription factor GLI1 identifies a signaling network in the tumor microenvironment mediating KRAS oncogene-induced transformation.
    Authors: Mills, Lisa D, Zhang, Yaqing, Marler, Ronald J, Herreros-Villanueva, Marta, Zhang, Lizhi, Almada, Luciana, Couch, Fergus, Wetmore, Cynthia, Pasca di Magliano, Marina, Fernandez-Zapico, Martin E
    J Biol Chem, 2013-03-12;288(17):11786-94.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  21. Heparan sulfate-modulated, metalloprotease-mediated sonic hedgehog release from producing cells.
    Authors: Dierker T, Dreier R, Petersen A, Bordych C, Grobe K
    J. Biol. Chem., 2009-01-27;284(12):8013-22.
    Species: Mouse
    Sample Types: Recombinant Protein
    Applications: Western Blot
  22. Involvement of the Edar signaling in the control of hair follicle involution (catagen).
    Authors: Fessing MY, Sharova TY, Sharov AA, Atoyan R, Botchkarev VA
    Am. J. Pathol., 2006-12-01;169(6):2075-84.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  23. Neuroprotective properties of cultured neural progenitor cells are associated with the production of sonic hedgehog.
    Authors: Rafuse VF, Soundararajan P, Leopold C, Robertson HA
    Neuroscience, 2005-01-01;131(4):899-916.
    Species: Chicken, Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  24. Sonic hedgehog exerts distinct, stage-specific effects on tongue and taste papilla development.
    Authors: Liu HX, MacCallum DK, Edwards C, Gaffield W, Mistretta CM
    Dev. Biol., 2004-12-15;276(2):280-300.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  25. Cyclopamine and jervine in embryonic rat tongue cultures demonstrate a role for Shh signaling in taste papilla development and patterning: fungiform papillae double in number and form in novel locations in dorsal lingual epithelium.
    Authors: Mistretta CM, Liu HX, Gaffield W, MacCallum DK
    Dev. Biol., 2003-02-01;254(1):1-18.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  26. Sonic hedgehog regulates gastric gland morphogenesis in man and mouse.
    Authors: van den Brink GR, Hardwick JC, Tytgat GN, Brink MA, Ten Kate FJ, Van Deventer SJ, Peppelenbosch MP
    Gastroenterology, 2001-08-01;121(2):317-28.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  27. Probing the multimodal fungiform papilla: complex peripheral nerve endings of chorda tympani taste and mechanosensitive fibers before and after Hedgehog pathway inhibition
    Authors: Donnelly CR, Kumari A, Li L et al.
    Cell and Tissue Research
  28. Sonic hedgehog promotes autophagy of vascular smooth muscle cells
    Authors: Haijie Li, Jingjing Li, Yuenan Li, Pavneet Singh, Liang Cao, Li-juan Xu et al.
    American Journal of Physiology-Heart and Circulatory Physiology
  29. Interaction between hedgehog signalling and PAX6 dosage mediates maintenance and regeneration of the corneal epithelium
    Authors: Romana Kucerova, Natalie Dorà, Richard L. Mort, Karen Wallace, Lucy J. Leiper, Christina Lowes et al.
    Mol Vis
  30. Sonic Hedgehog acts as a macrophage chemoattractant during regeneration of the gastric epithelium
    Authors: Chakrabarti J, Dua-Awereh M, Schumacher M et al.
    NPJ Regenerative medicine
  31. Increased activity of mesenchymal ALK2‐BMP signaling causes posteriorly truncated microglossia and disorganization of lingual tissues
    Authors: Mohamed Ishan, Guiqian Chen, Chenming Sun, Shi‐You Chen, Yoshihiro Komatsu, Yuji Mishina et al.
    genesis
  32. Increased Programmed Death-Ligand 1 is an Early Epithelial Cell Response to Helicobacter pylori Infection
    Authors: Holokai L, Chakrabarti J, Broda T et al.
    PLoS Pathog.
  33. Separate and distinctive roles for Wnt5a in tongue, lingual tissue and taste papilla development
    Authors: Hong-Xiang Liu, Ann S. Grosse, Ken Iwatsuki, Yuji Mishina, Deborah L. Gumucio, Charlotte M. Mistretta
    Developmental Biology

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Human/Mouse Sonic Hedgehog/Shh N‑Terminus Antibody
By Anonymous on 12/14/2018
Application: Immunocytochemistry/Immunofluorescence Sample Tested: Embryonic spinal cord,Embryonic stem cells Species: Human

H9 cells were fixed in 4%PFA. Nanog antibody (15ug/ml) was incubated overnight at 4 °C. Secondary antibody was donkey anti- goat 488 (Invitrogen).

Immunocytochemistry/Immunofluorescence Human/Mouse Sonic Hedgehog/Shh N‑Terminus Antibody AF464
Human/Mouse Sonic Hedgehog/Shh N‑Terminus Antibody
By Anonymous on 12/14/2018
Application: Immunocytochemistry/Immunofluorescence Sample Tested: Embryo spinal cord,Spinal cord Species: Mouse

Frozen section. shh antibody (15ug/ml) incubated at 15 µg/mL overnight at 4 °C

Immunocytochemistry/Immunofluorescence Human/Mouse Sonic Hedgehog/Shh N‑Terminus Antibody AF464