Human/Mouse SSEA-3 Alexa Fluor® 594-conjugated Antibody

Catalog #: FAB1434T Datasheet / COA / SDS

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FAB1434T-100UG

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Human/Mouse SSEA-3 Alexa Fluor® 594-conjugated Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Recognizes a carbohydrate epitope of SSEA-3 (4, 5).

Source

Monoclonal Rat IgM Clone # MC-631

Immunogen

Four to eight cell stage mouse embryos

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Label

Alexa Fluor 594 (Excitation= 590 nm, Emission= 617 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25-1 µg/10⁶ cells

D3 mouse embryonic stem cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: SSEA-3

SSEA-3, also known as glycolipid GB5, is expressed on the surface of human teratocarcinoma stem cells (EC), human embryonic germ cells (EG), and human embryonic stem cells (ES) (1). Expression of SSEA-3 is down regulated following differentiation of human EC cells. In contrast, the differentiation of murine EC and ES cells may be accompanied by an increase in SSEA-3 expression (2, 3).

References

Zhou, D. et al. (2000) J. Biol. Chem. 275:22631.
Thomson, J.A. and J.S. Odorico (2000) Trends Biotechnol. 18:53.
Draper, J.S. et al. (2002) J. Anat. 200:249.
Shevinsky, L.H. et al. (1982) Cell 30:697.
Kannagi, R. et al. (1983) EMBO J. 2:2355.

Long Name

Stage-specific Embryonic Antigen-3

Alternate Names

SSEA3; SSEA-3; Stage specific embryonic antigen 3; Stage-specific embryonic antigen 3

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Product Specific Notices

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.