Human/Mouse TREM2 APC-conjugated Antibody Summary
extracellular domain
Accession # Q99NH8
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of TREM‑2 in RAW 264.7 Mouse Cell Line by Flow Cytometry. RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Human/Mouse TREM-2 APC-conjugated Monoclonal Antibody (Catalog # FAB17291A, filled histogram) or isotype control antibody (Catalog # IC013A, open histogram). View our protocol for Staining Membrane-associated Proteins.
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Detection of TREM‑2 in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with (A) Rat Anti-Human/Mouse TREM-2 APC-conjugated Monoclonal Antibody (Catalog # FAB17291A) or (B) isotype control antibody (Catalog # IC013A) followed by Mouse anti-Human Integrin alpha M/CD11b PE-conjugated Monoclonal Antibody (Catalog # FAB16991P). View our protocol for Staining Membrane-associated Proteins.
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Detection of TREM‑2 in HEK Human Cell Line Transfected with Human TREM-2 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) human TREM-2 or (B) human TREM-1 and eGFP was stained with Rat Anti-Human/Mouse TREM-2 APC-conjugated Monoclonal Antibody (Catalog # FAB17291A). Quadrant markers were set based on control antibody staining (Catalog # IC013A). View our protocol for Staining Membrane-associated Proteins.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: TREM2
TREM-2 (Triggering Receptor Expressed on Myeloid cells-2) is a 35 kDa molecular weight type I transmembrane member of the TREM family and Ig superfamily. Mature human TREM-2 consists of a 156 amino acid (aa) extracellular domain (ECD) with one V-type Ig-like domain, a 21 aa transmembrane (TM) domain, and a 35 aa cytoplasmic tail. Within the ECD, human TREM-2 shares 73% and 74% aa sequence identity with mouse and rat TREM-2, respectively. Two closely related transcripts were reported in mouse and designated TREM-2a and TREM-2b. Soluble forms of the TREM-2 ECD are generated by alternative splicing or proteolytic cleavage, and the cytoplasmic domain can be liberated by gamma-Secretase mediated intramembrane cleavage. It is a pattern recognition receptor that binds anionic ligands. A positively charged lysine within the transmembrane segment allows association with the signal adapter protein, DAP12 to deliver an activating signal that plays a role in both innate and adaptive immune responses, including inhibition of macrophage activation. TREM-2 is expressed on macrophages, immature myeloid dendritic cells, osteoclasts, microglia, and adipocytes. It promotes the differentiation and function of osteoclasts, the production of inflammatory cytokines by adipocytes, insulin resistance, and the phagocytic clearance of bacteria. In the CNS, TREM-2 binds to ApoE, ApoA1, and ApoB and mediates the clearance of apoptotic neurons, amyloid plaques, and cell debris following demyelination. TREM-2 also interacts with and modifies signaling through Plexin A1 on dendritic cells and osteoclasts. Mutations in TREM-2 or DAP12 are associated with the development of Alzheimer's disease and Nasu-Hakola disease (NHD/PLOSL) which is characterized by presenile dementia and bone cysts. Soluble TREM-2 is elevated in cerebrospinal fluid of patients with active multiple sclerosis (MS), and TREM-2 blockade exacerbates disease symptoms in the experimental EAE model of MS.
Product Datasheets
Citations for Human/Mouse TREM2 APC-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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TREM2 macrophages induced by human lipids drive inflammation in acne lesions
Authors: Do TH, Ma F, Andrade PR et al.
Science immunology
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Analysis of Intracellular Communication Reveals Consistent Gene Changes Associated with Early-Stage Acne Skin
Authors: Deng, M;Odhiambo, WO;Qin, M;To, TT;Brewer, GM;Kheshvadjian, AR;Cheng, C;Agak, GW;
Research square
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Neutral ceramidase regulates breast cancer progression by metabolic programming of TREM2-associated macrophages
Authors: Sun, R;Lei, C;Xu, Z;Gu, X;Huang, L;Chen, L;Tan, Y;Peng, M;Yaddanapudi, K;Siskind, L;Kong, M;Mitchell, R;Yan, J;Deng, Z;
Nature communications
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
A novel phenotype of B cells associated with enhanced phagocytic capability and chemotactic function after ischemic stroke
Authors: Rui Wang, Huaming Li, Chenhan Ling, Xiaotao Zhang, Jianan Lu, Weimin Luan et al.
Neural Regeneration Research
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Distinct myeloid population phenotypes dependent on TREM2 expression levels shape the pathology of traumatic versus demyelinating CNS disorders
Authors: Gao, H;Di, J;Clausen, BH;Wang, N;Zhu, X;Zhao, T;Chang, Y;Pang, M;Yang, Y;He, R;Wang, Y;Zhang, L;Liu, B;Qiu, W;Lambertsen, KL;Brambilla, R;Rong, L;
Cell reports
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Skin basal cell carcinomas assemble a pro-tumorigenic spatially organized and self-propagating Trem2+ myeloid niche
Authors: Haensel, D;Daniel, B;Gaddam, S;Pan, C;Fabo, T;Bjelajac, J;Jussila, AR;Gonzalez, F;Li, NY;Chen, Y;Hou, J;Patel, T;Aasi, S;Satpathy, AT;Oro, AE;
Nature communications
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
The correlation between triiodothyronine and the severity of liver fibrosis
Authors: W He, C Huang, L Wang, W Su, S Wang, P Huang, X Zhang, Y Huang, Y Zhao, M Lin, X Shi, X Li
BMC endocrine disorders, 2022-12-12;22(1):313.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Early life stress impairs synaptic pruning in the developing hippocampus
Authors: KK Dayananda, S Ahmed, D Wang, B Polis, R Islam, A Kaffman
Brain, Behavior, and Immunity, 2022-09-26;107(0):16-31.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
A single-cell atlas of the multicellular ecosystem of primary and metastatic hepatocellular carcinoma
Authors: Y Lu, A Yang, C Quan, Y Pan, H Zhang, Y Li, C Gao, H Lu, X Wang, P Cao, H Chen, S Lu, G Zhou
Nature Communications, 2022-08-06;13(1):4594.
Species: Human
Sample Types: Tissue Homogenates
Applications: Flow Cytometry -
Spatiotemporal dynamics of macrophage heterogeneity and a potential function of Trem2hi macrophages in infarcted hearts
Authors: SH Jung, BH Hwang, S Shin, EH Park, SH Park, CW Kim, E Kim, E Choo, IJ Choi, FK Swirski, K Chang, YJ Chung
Nature Communications, 2022-08-06;13(1):4580.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
TREM2 Promotes Immune Evasion by Mycobacterium tuberculosis in Human Macrophages
Authors: A Dabla, YC Liang, N Rajabalee, C Irwin, CGJ Moonen, JV Willis, S Berton, J Sun
MBio, 2022-08-04;13(4):e0145622.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Isolation of macrophages from mouse skin wounds for single-cell RNA sequencing
Authors: Sebastian Willenborg, Juliana G. Roscito, Alexander Gerbaulet, Axel Roers, Andreas Dahl, Sabine A. Eming et al.
STAR Protocols
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GW5074 Increases Microglial Phagocytic Activities: Potential Therapeutic Direction for Alzheimer’s Disease
Authors: Sarah M. Connor, Mamunur Rashid, Katie J. Ryan, Kruti Patel, Justin D. Boyd, Jennifer Smith et al.
Frontiers in Cellular Neuroscience
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Discovering dominant tumor immune archetypes in a pan-cancer census
Authors: Alexis J. Combes, Bushra Samad, Jessica Tsui, Nayvin W. Chew, Peter Yan, Gabriella C. Reeder et al.
Cell
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Targeting TREM2 on tumor-associated macrophages enhances immunotherapy
Authors: M Binnewies, JL Pollack, J Rudolph, S Dash, M Abushawish, T Lee, NS Jahchan, P Canaday, E Lu, M Norng, S Mankikar, VM Liu, X Du, A Chen, R Mehta, R Palmer, V Juric, L Liang, KP Baker, L Reyno, MF Krummel, M Streuli, V Sriram
Cell Reports, 2021-10-19;37(3):109844.
Species: Human
Sample Types: Whole Cell
Applications: Flow Cytometry -
Differential expression of Triggering Receptor Expressed on Myeloid cells 2 (Trem2) in tissue eosinophils
Authors: AC Sek, CM Percopo, AK Boddapati, M Ma, WE Geslewitz, JO Krumholz, JB Lack, HF Rosenberg
Journal of leukocyte biology, 2021-01-06;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Decidual glycodelin-A polarizes human monocytes towards a decidual macrophage-like phenotype via siglec-7
Authors: M Vijayan, CL Lee, VHH Wong, X Wang, K Bai, J Wu, H Koistinen, M Seppälä, KF Lee, WSB Yeung, EHY Ng, PCN Chiu
J. Cell. Sci., 2020-07-23;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
TREM-1 and TREM-2 Expression on Blood Monocytes Could Help Predict Survival in High-Grade Glioma Patients
Authors: K Kluckova, J Kozak, K Szaboova, B Rychly, M Svajdler, M Suchankova, E Tibenska, B Filova, J Steno, V Matejcik, M Homolova, M Bucova
Mediators Inflamm., 2020-07-01;2020(0):1798147.
Species: Human
Sample Types: Plasma, Whole Blood
Applications: Flow Cytometry -
A CX3CR1 Reporter hESC Line Facilitates Integrative Analysis of In-Vitro-Derived Microglia and Improved Microglia Identity upon Neuron-Glia Co-culture
Authors: A Grubman, TH Vandekolk, J Schröder, G Sun, J Hatwell-Hu, J Chan, M Oksanen, S Lehtonen, C Hunt, JE Koistinaho, SK Nilsson, JM Haynes, CW Pouton, JM Polo
Stem Cell Reports, 2020-05-21;14(6):1018-1032.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
TREM-1 and TREM-2 Expression on CD14+ Cells in Bronchoalveolar Lavage Fluid in Pulmonary Sarcoidosis and Hypersensitivity Pneumonitis in the Context of T Cell Immune Response
Authors: M Suchankova, J Urban, M Ganovska, E Tibenska, K Szaboova, E Tedlova, F Sandor, I Majer, M Bobovcak, I Jonner, B Konig, M Bucova
Mediators Inflamm., 2020-05-14;2020(0):9501617.
Species: Human
Sample Types: Bronchoalveolar lavage fluid
Applications: Flow Cytometry -
Unleashing Type-2 Dendritic Cells to Drive Protective Antitumor CD4+ T Cell Immunity
Authors: Mikhail Binnewies, Adriana M. Mujal, Joshua L. Pollack, Alexis J. Combes, Emily A. Hardison, Kevin C. Barry et al.
Cell
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TREM2-activating antibodies abrogate the negative pleiotropic effects of the Alzheimer's disease variant TREM2R47Hon murine myeloid cell function
Authors: Q Cheng, J Danao, S Talreja, P Wen, J Yin, N Sun, CM Li, D Chui, D Tran, S Koirala, H Chen, IN Foltz, S Wang, S Sambashiva
J. Biol. Chem., 2018-03-29;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Increased TREM-2 expression on the subsets of CD11c(+) cells in the lungs and lymph nodes during allergic airway inflammation
Authors: SC Hall, DK Agrawal
Sci Rep, 2017-09-19;7(1):11853.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Flow Cytometry -
CD33 modulates TREM2: convergence of Alzheimer loci.
Authors: Chan G, White C, Winn P, Cimpean M, Replogle J, Glick L, Cuerdon N, Ryan K, Johnson K, Schneider J, Bennett D, Chibnik L, Sperling R, Bradshaw E, De Jager P
Nat Neurosci, 2015-09-28;18(11):1556-8.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
FAQs
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What type of light chain does the Human/Mouse TREM-2 Antibody, Cat# MAB17291, clone # 237920 have?
This antibody has a kappa light chain.
Reviews for Human/Mouse TREM2 APC-conjugated Antibody
Average Rating: 4 (Based on 2 Reviews)
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Analysis of TREM2 surface expression on human dendritic cells
