Human/Mouse TRIM32 Antibody

Catalog #: AF6515
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF6515
AF6515-SP

Detection of Human and Mouse TRIM32 by Western Blot.

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All TRIM32 Antibodies

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Citations (1)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse TRIM32 Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects human and mouse TRIM32 in Western blots.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human TRIM32
Arg105-Lys204
Accession # Q13049

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.5 µg/mL

See below

Immunohistochemistry

5-15 µg/mL

See below

Immunocytochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human and Mouse TRIM32 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, PC-3 human prostate cancer cell line, and A20 mouse B cell lymphoma cell line. PVDF Membrane was probed with 0.5 µg/mL of Sheep Anti-Human/Mouse TRIM32 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6515) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for TRIM32 at approximately 72 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Immunocytochemistry

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TRIM32 in HeLa Human Cell Line. TRIM32 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Sheep Anti-Human/Mouse TRIM32 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6515) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry

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TRIM32 in Human Prostate Cancer Tissue. TRIM32 was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using Sheep Anti-Human/Mouse TRIM32 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6515) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TRIM32

TRIM32 (Tripartite motif-containing protein 32; also 72 kDa TAT-interacting protein, and zinc finger protein HT2A) is a 72-82 kDa member of the TRIM/RBCC family of proteins. It is an E3 ligase that is found in structures termed nuclear and cytoplasmic bodies. Cells known to express TRIM32 are diverse and include fibroblasts, keratinocytes, skeletal muscle cells and neurons. TRIM32 ubiquitinates select proteins such as c-myc, Abi2, actin and dysbindin. Human TRIM32 is 653 amino acids (aa) in length. It contains one E3 ligase RING finger domain (aa 20-65), a B-Box type zinc-finger region (aa 103-133), a coiled-coil region (aa 138-197), five NHL repeats (aa 358-646) and three utilized phosphorylation sites (Ser328/335/339). TRIM32 has the potential to form homomultimers. Over aa 105-204, human TRIM32 exhibits 95% aa identity with mouse TRIM32.

Long Name

Tripartite Motif-containing Protein 32

Entrez Gene IDs

22954 (Human); 69807 (Mouse); 313264 (Rat)

Alternate Names

72 kDa Tat-interacting protein; BBS11; BBS1172-KD; EC 6.3.2.-; HT2A; HT2Alimb girdle muscular dystrophy 2H (autosomal recessive); LGMD2H; TATIP; TRIM32; tripartite motif containing 32; Zinc finger protein HT2A; zinc-finger protein HT2A

Product Datasheets

Product Datasheet

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Related Research Areas

Citation for Human/Mouse TRIM32 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Non-proteolytic ubiquitination of OTULIN regulates NF-kappa B signaling pathway
Authors: Mengmeng Zhao, Kun Song, Wenzhuo Hao, Lingyan Wang, Girish Patil, Qingmei Li et al.
Journal of Molecular Cell Biology