Human/Mouse TSG-6 Antibody

Catalog # Availability Size / Price Qty
MAB2104
MAB2104-SP
Product Details
Citations (6)
FAQs
Supplemental Products
Reviews (8)

Human/Mouse TSG-6 Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human and mouse TSG-6 in direct ELISAs and Western blots.
Source
Monoclonal Mouse IgG2B Clone # 259820
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human TSG-6
Trp18-Leu277
Accession # P98066
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human TSG‑6 (Catalog # 2104-TS)
Recombinant Mouse TSG‑6 (Catalog # 2326-TS)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TSG-6

TSG-6 is a secreted hyaluronan-binding protein composed of a link domain and Cub domain. It is induced in many cell types by inflammatory mediators. Via its link domain, TSG-6 is a potent inhibitor of polymorphonuclear leukocyte migration. TSG-6 forms a stable complex with the serine protease inhibitor Inter-alpha -Inhibitor (I alpha I) and potentiates the anti-plasmin activity of I alpha I.

Long Name
Tumor Necrosis Factor-stimulated Gene Sequence 6
Entrez Gene IDs
7130 (Human); 21930 (Mouse)
Alternate Names
Hyaluronate-binding protein; TNF alpha-induced protein 6; TNFAIP6; TNF-stimulated gene 6 protein; TSG6; TSG-6; TSG-6tumor necrosis factor alpha-inducible protein 6; TSG6tumor necrosis factor-inducible gene 6 protein; Tumor necrosis factor alpha-induced protein 6; tumor necrosis factor, alpha-induced protein 6; tumor necrosis factor-stimulated gene-6 protein

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Citations for Human/Mouse TSG-6 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. TSG-6+ cancer-associated fibroblasts modulate myeloid cell responses and impair anti-tumor response to immune checkpoint therapy in pancreatic cancer
    Authors: Anandhan, S;Herbrich, S;Goswami, S;Guan, B;Chen, Y;Macaluso, MD;Jindal, S;Natarajan, SM;Andrewes, SW;Xiong, L;Nagarajan, A;Basu, S;Tang, DN;Liu, J;Min, J;Maitra, A;Sharma, P;
    Nature communications
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  2. Prostaglandin E2 Indicates Therapeutic Efficacy Of Mesenchymal Stem Cells In Experimental Traumatic Brain Injury
    Authors: DJ Kota, KS Prabhakara, N Toledano-F, D Bhattarai, Q Chen, B DiCarlo, P Smith, F Triolo, PL Wenzel, CS Cox, SD Olson
    Stem Cells, 2017-03-16;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. TNF?-stimulated gene-6 (TSG6) activates macrophage phenotype transition to prevent inflammatory lung injury
    Proc. Natl. Acad. Sci. U.S.A., 2016-11-28;113(50):E8151-E8158.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. Endogenous mesenchymal stromal cells in bone marrow are required to preserve muscle function in mdx mice.
    Authors: Fujita R, Tamai K, Aikawa E, Nimura K, Ishino S, Kikuchi Y, Kaneda Y
    Stem Cells, 2015-03-01;33(3):962-75.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  5. Constitutive expression of inter-alpha-inhibitor (IalphaI) family proteins and tumor necrosis factor-stimulated gene-6 (TSG-6) by human amniotic membrane epithelial and stromal cells supporting formation of the heavy chain-hyaluronan (HC-HA) complex.
    Authors: Zhang S, He H, Day AJ, Tseng SC
    J. Biol. Chem., 2012-02-20;287(15):12433-44.
    Species: Human
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC-Fr, Western Blot
  6. Biochemical characterization and function of complexes formed by hyaluronan and the heavy chains of inter-alpha-inhibitor (HC*HA) purified from extracts of human amniotic membrane.
    Authors: He H, Li W, Tseng DY, Zhang S, Chen SY, Day AJ, Tseng SC
    J. Biol. Chem., 2009-06-02;284(30):20136-46.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Western Blot

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Reviews for Human/Mouse TSG-6 Antibody

Average Rating: 4.1 (Based on 8 Reviews)

5 Star
25%
4 Star
62.5%
3 Star
12.5%
2 Star
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1 Star
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Human/Mouse TSG-6 Antibody
By Ricardo Malvicini on 09/10/2021
Application: WB Sample Tested: extracellular vesicles Species: Human

Human/Mouse TSG-6 Antibody
By Anonymous on 09/01/2021
Application: WB Sample Tested: Plasma Species: Mouse

Human/Mouse TSG-6 Antibody
By Anonymous on 08/06/2021
Application: WB Sample Tested: Brain (cerebral cortex) Species: Mouse

this antibody was detected in the mouse cortex at different timepoint. It works very good with very clear band.


Human/Mouse TSG-6 Antibody
By Anonymous on 03/11/2019
Application: Microarrays Sample Tested: EDTA Plasma Species: Human

Human/Mouse TSG-6 Antibody
By Anonymous on 02/21/2019
Application: MiAr Sample Tested: EDTA Plasma Species: Human

Human/Mouse TSG-6 Antibody
By Anonymous on 01/19/2019
Application: WB Sample Tested: HUVEC human umbilical vein endothelial cells Species: Human

Western Blot with diff concentration


Human/Mouse TSG-6 Antibody
By Simon Liu on 12/08/2018
Application: IHC Sample Tested: HUVEC human umbilical vein endothelial cells Species: Human

Human/Mouse TSG-6 Antibody
By Anonymous on 11/11/2018
Application: ELISA Sample Tested: Serum and Plasma Species: Human