Human/Mouse VGF Antibody

Name: Human/Mouse VGF Antibody
Brand: R&D Systems
SKU: MAB6317
Rating: 5 (1 reviews)

Catalog #: MAB6317 1 Review Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB6317
MAB6317-SP

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Human/Mouse VGF Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects human and mouse VGF in direct ELISAs.

Source

Monoclonal Mouse IgG₃ Clone # 619707

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human VGF
Thr554-Pro615
Accession # O15240

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunohistochemistry

1.7-25 µg/mL

Immersion fixed paraffin-embedded sections of human brain (hypothalamus) and perfusion fixed frozen sections of mouse brain (cortex)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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VGF in Human Brain. VGF was detected in immersion fixed paraffin-embedded sections of human brain (hypothalamus) using Mouse Anti-Human/Mouse VGF Monoclonal Antibody (Catalog # MAB6317) at 1.7 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in neurons. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VGF

Neurosecretory protein VGF (VGF) is a 65 kDa secreted protein that is expressed in the brain, where it may be involved in the regulation of cell-cell interactions or in synaptogenesis during maturation of the nervous system. Human VGF is synthesized as a 615 amino acid (aa) precursor that contains a 22 aa signal sequence and a 593 aa mature chain. The region including aa 353-447 is Asp/Glu-rich and thus an acidic region. Human VGF shares 87% aa sequence identity to mouse VGF.

Long Name

Neurosecretory Protein VGF

Entrez Gene IDs

7425 (Human)

Alternate Names

neuro-endocrine specific protein VGF; neurosecretory protein VGF; VGF nerve growth factor inducible; VGF