Human/Mouse WASF1/WAVE1 Antibody Summary
Met1-Tyr250
Accession # Q92558
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human/Mouse WASF1/WAVE1 by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line, A172 human glioblastoma cell line, and 293T human embryonic kidney cell line, and mouse brain tissue. PVDF membrane was probed with 1 µg/mL of Human/Mouse WASF1/WAVE1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5514) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for WASF1/WAVE1 at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: WASF1/WAVE1
WASF1/WAVE1 (Wiscott-Aldrich syndrome protein family Verprolin-homologous protein 1) is an 80‑85 kDa member of the SCAR/WAVE family of proteins. It is expressed in diverse cell types such as neurons, fibroblasts, macrophages, platelets, oligodendroglia and breast epithelium. WASF1/WAVE1 is normally inactive, being phosphorylated at Ser310 and complexed with HSPC300, PIR121 and Nap125. Upon activation, it is dephosphorylated and decoupled, heterodimerizes with WASP, and initiates actin polymerization. Human WASF1/WAVE1 is 559 amino acids (aa) in length. It contains five sequential poly-Pro regions (aa 278‑435) and one VPH domain (aa 497‑514) that binds to actin and Arp2/3. There is one isoform that shows a 23 aa N-terminal extension. In mouse, a 60 kDa proteolytic cleavage form is reported. Over aa 1‑250, human WASF1/WAVE1 shares 99% aa identity with mouse WASF1/WAVE1.
Product Datasheets
Citations for Human/Mouse WASF1/WAVE1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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PPP2R1A regulates migration persistence through the NHSL1-containing WAVE Shell Complex
Authors: Wang, Y;Chiappetta, G;Guérois, R;Liu, Y;Romero, S;Boesch, DJ;Krause, M;Dessalles, CA;Babataheri, A;Barakat, AI;Chen, B;Vinh, J;Polesskaya, A;Gautreau, AM;
Nature communications
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
A Rac1-FMNL2 signaling module affects cell-cell contact formation independent of Cdc42 and membrane protrusions
Authors: H Grobe, A Wüstenhage, C Baarlink, R Grosse, K Grikscheit
PLoS ONE, 2018-03-26;13(3):e0194716.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
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