Human/Mouse WASF3/WAVE3 Antibody Summary
Met1-Lys180
Accession # Q9UPY6
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
View Larger
Detection of Human WASF3/WAVE3 by Western Blot. Western blot shows lysates of A172 human glioblastoma cell line, SH-SY5Y human neuroblastoma cell line, and LNCaP human prostate cancer cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse WASF3/WAVE3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5515) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for WASF3/WAVE3 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: WASF3/WAVE3
WASF3/WAVE3 (Wiscott-Aldrich syndrome protein family Verprolin-homologous protein 3) is a 70 kDa member of the SCAR/WAVE family of proteins. It is expressed in select diverse cell types such as neurons, platelets, and breast epithelium. Upon activation, WASF3/WAVE3 is phosphorylated on Tyr151/248/337/486 and, in complex with HSPC300, initiates actin polymerization. WASF3/WAVE3 also regulates MMP expression. Human WASF3/WAVE3 is 502 amino acids (aa) in length. It contains one coiled-coil region (aa 57‑93), three sequential poly-Pro regions (aa 304‑410) and one VPH domain (aa 440‑457) that binds to actin and Arp2/3. There is one isoform that shows a 36 aa substitution for aa 181‑239. Over aa 1‑180, human WASF3 shares 98% aa identity with mouse WASF3.
Product Datasheets
Citations for Human/Mouse WASF3/WAVE3 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
2
Citations: Showing 1 - 2
Filter your results:
Filter by:
-
PPP2R1A regulates migration persistence through the NHSL1-containing WAVE Shell Complex
Authors: Wang, Y;Chiappetta, G;Guérois, R;Liu, Y;Romero, S;Boesch, DJ;Krause, M;Dessalles, CA;Babataheri, A;Barakat, AI;Chen, B;Vinh, J;Polesskaya, A;Gautreau, AM;
Nature communications
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
PCARE requires coiled coil, RP62 kinase-binding and EVH1 domain-binding motifs for ciliary expansion
Authors: Tess A V Afanasyeva, Yan-Ting Schnellbach, Toby J Gibson, Ronald Roepman, Rob W J Collin
Human Molecular Genetics
FAQs
No product specific FAQs exist for this product, however you may
View all Antibody FAQsReviews for Human/Mouse WASF3/WAVE3 Antibody
There are currently no reviews for this product. Be the first to review Human/Mouse WASF3/WAVE3 Antibody and earn rewards!
Have you used Human/Mouse WASF3/WAVE3 Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
