Human/Mouse Wnt-4 Antibody

Catalog #: MAB475
3 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB475-050
MAB475-500
MAB475-SP

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All Wnt-4 Antibodies

Product Details

Citations (3)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse Wnt-4 Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects mouse Wnt-4 in direct ELISAs and Western blots. In sandwich immunoassays, this antibody is specific for human Wnt-4 as a capture antibody when paired with Sheep Anti-Human Wnt‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6076)

Source

Monoclonal Rat IgG_2A Clone # 55010

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant mouse Wnt-4
aa 37-76 and 222-295
Accession # P22724

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Mouse Wnt‑4 (Catalog # 475-WN)

ELISA

This antibody functions as an ELISA capture antibody for Human Wnt-4 when paired with Sheep Anti-Human Wnt‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6076).

This product is intended for assay development on various assay platforms requiring antibody pairs.

Immunohistochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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Wnt‑4 in Mouse Embryo. Wnt-4 was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c.) using Rat Anti-Mouse Wnt-4 Monoclonal Antibody (Catalog # MAB475) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to muscle cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

ELISA

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Human Wnt-4 ELISA Standard Curve. Recombinant Human Wnt-4 protein was serially diluted 2-fold and captured by Rat Anti-Human/Mouse Wnt-4 Monoclonal Antibody (Catalog # MAB475) coated on a Clear Polystyrene Microplate (DY990). Sheep Anti-Human Wnt-4 Antigen Affinity-purified Polyclonal Antibody (AF6076) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Wnt-4

Wnt-4 is a secreted glycoprotein that binds to receptors of the Frizzled family. Wnt-4 is important for multiple embryonic development processes including kidney morphogenesis.

Long Name

Wingless-type MMTV Integration Site Family, Member 4

Entrez Gene IDs

54361 (Human); 22417 (Mouse)

Alternate Names

protein Wnt-4; SERKAL; wingless-type MMTV integration site family, member 4; Wnt4; Wnt-4

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human/Mouse Wnt-4 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Macrophages promote epithelial proliferation following infectious and non-infectious lung injury through a Trefoil factor 2-dependent mechanism
Authors: LY Hung, D Sen, TK Oniskey, J Katzen, NA Cohen, AE Vaughan, W Nieves, A Urisman, MF Beers, MF Krummel, DR Herbert
Mucosal Immunol, 2018-10-18;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization
Wnt signaling in the pathogenesis of human HIV-associated pain syndromes.
Authors: Shi, Yuqiang, Shu, Jianhong, Gelman, Benjamin, Lisinicchia, Joshua G, Tang, Shao-Jun
J Neuroimmune Pharmacol, 2013-06-05;8(4):956-64.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
Wnt glycoproteins regulate the expression of FoxN1, the gene defective in nude mice.
Authors: Balciunaite G, Keller MP, Balciunaite E, Piali L, Zuklys S, Mathieu YD, Gill J, Boyd R, Sussman DJ, Hollander GA
Nat. Immunol., 2002-10-15;3(11):1102-8.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC