Human/Mouse YY1 Antibody

Catalog #: MAB3784
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB3784
MAB3784-SP

Detection of Human and Mouse YY1 by Western Blot.

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All YY1 Antibodies

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Citations (1)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse YY1 Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects human YY1 in ELISAs. In direct ELISAs, no cross-reactivity with recombinant human Rex-1/ZFP42 is observed.

Source

Monoclonal Mouse IgG_2B Clone # 854847

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human YY1
His75-Gln275
Accession # P25490

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

0.5 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human and Mouse YY1 by Western Blot. Western blot shows lysates of U937 human histiocytic lymphoma cell line and C2C12 mouse myoblast cell line. Gels were loaded with 30 µg of U937 and 40 µg of C2C12 cytoplasmic (Cyto) extracts and 15 µg of U937 and 20 µg of C2C12 nuclear (Nuc) extracts. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse YY1 Monoclonal Antibody (Catalog # MAB3784) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for YY1 at approximately 60-65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: YY1

YY1 (Yin Yang 1) is a multifunctional transcription factor belonging to the GLI-Kruppel class of zing finger proteins. YY1 is essential for embryogenesis, apoptosis, differentiation, cell growth and proliferation. YY1 binds to the DNA consensus site CGCCATNTT. It can function as an initiator of transcription as well as activating or repressing transcription through protein complexes (e.g. histone deacetylases and histone acetyltransferases).

Long Name

Yin-Yang-1

Entrez Gene IDs

7528 (Human); 22632 (Mouse); 24919 (Rat)

Alternate Names

Delta transcription factor; DELTA; INO80SYY-1; NF-E1; NF-E1INO80 complex subunit S; transcriptional repressor protein YY1; UCRBP; Yin and Yang 1 protein; Yin and yang 1; YIN-YANG-1; YY1 transcription factor; YY1

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Human/Mouse YY1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Meteorin links the bone marrow hypoxic state to hematopoietic stem/progenitor cell mobilization
Authors: YW Dai, JK Ma, R Jiang, XL Zhan, SY Chen, LL Feng, Q Zhang, TB Liang, K Lv, GJ Yang, JF Lu, J Chen, XJ Lu
Cell Reports, 2022-09-20;40(12):111361.
Species: Human, Mouse
Sample Types: Cell Lysates
Applications: Western Blot