Human Mrc2 Antibody

Catalog #: MAB5770 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB5770-100
MAB5770-SP

Detection of Mrc2 in THP‑1 cells (positive) and A431 cells (negative).

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Mrc2 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Mrc2 in direct ELISA.

Source

Monoclonal Mouse IgG_2B Clone # 1063704

Purification

Protein A or G purified from cell culture supernatant

Immunogen

Mouse myeloma cell line, NS0-derived human Mrc2
Gly31-Ala1414
Accession # Q9UBG0

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

THP‑1 human acute monocytic leukemia cell line

Immunocytochemistry

8-25 µg/mL

Immersion fixed THP‑1 human acute monocytic leukemia cells (positive) and A431 human epithelial carcinoma cells (negative)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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Detection of Mrc2 in THP‑1 cells (positive) and A431 cells (negative). Mrc2 was detected in immersion fixed THP‑1 human acute monocytic leukemia cells (positive) and absent in A431 human epithelial carcinoma cells (negative) using Mouse Anti-Human Mrc2 Monoclonal Antibody (Catalog # MAB5770) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Flow Cytometry

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Detection of Mrc2 in THP-1 cells by Flow Cytometry. THP-1 cells were stained with Mouse Anti-Human Mrc2 Monoclonal Antibody (Catalog # MAB5770, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Mrc2

Mrc2 (C-type Mannose Receptor 2), also know as MMR2, Endocytic Receptor 180 and uPARAP, is a 180‑kDa type I transmembrane protein. It is one of the mannose receptor (MR) family members which share a common domain organization and have a broad range of biological functions (1). Mrc2 is an endocytic receptor that is found on migrating cells, including cancer cells, macrophages, fibroblasts and endothelial cells (2). Mature human Mrc2 is composed of 1449 amino acid (aa) that includes a 1384 aa extracellular domain (ECD), a 21 aa transmembrane region, and a 44 aa cytoplasmic domain. The ECD shows one ricin B‑type lectin domain, one fibronectin type II domain and eight C‑type lectin domains. Within the ECD, human Mrc2 shares 91% aa identity with mouse and rat Mrc2. Mrc2 plays an important role in extracellular matrix remodeling through interaction with its ligands, including Man, Fuc, NAcGlc, collagens and urokinase plasminogen activator receptor (uPAR) (1-3). This cell surface molecule has been reported to promote cell invasion through matrix remodeling by internalizing large fragments of collagen and routing it to the lysosome for intracellular degradation and cell chemotaxis (2). It has also been reported to interact with matrix metalloprotease-13 (MMP-13) and collagen V on the cell surface (4).

References

Yuan, C. et al. (2016) Biochem. J. 473:2359.
Durrel, T. et al. (2018) Nat. Commun. 9:5178.
Behrendt, N. et al. (2000) J. Biol. Chem. 275:1993.
Englehom, L.H. et al. (2001) Lab. Invest. 81:1403.

Long Name

Mannose Receptor, C-type 2

Entrez Gene IDs

9902 (Human); 17534 (Mouse); 498011 (Rat)

Alternate Names

CD280 antigen; CD280; CD280C-type mannose receptor 2; CLEC13E; CLEC13EUPAR-associated protein; Endo180; ENDO180C-type lectin domain family 13 member E; Endocytic receptor 180; KIAA0709FLJ35911; Macrophage mannose receptor 2; mannose receptor, C type 2; Mrc2; uPARAP; UPARAPendocytic receptor (macrophage mannose receptor family); urokinase plasminogen activator receptor-associated protein; Urokinase receptor-associated protein; Urokinase-type plasminogen activator receptor-associated protein