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Human MRGPRF Antibody

Catalog #: MAB8396
1 Citation Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB8396
MAB8396-SP
Detection of Human MRGPRF by Western Blot.
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Product Details
Citations (1)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human MRGPRF Antibody Summary

Species Reactivity
Human
Specificity
Detects HEK293 human embryonic kidney cell line transfected with human MRGPRF by Flow Cytometry. Does not detect untransfected or irrelevant transfected HEK293 cells.
Source
Monoclonal Mouse IgG1 Clone # 916219
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with human MRGPRF
Met1-Ser343
Accession # NP_659452
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human MRGPRF antibody by Western Blot. View Larger

Detection of Human MRGPRF by Western Blot. Western blot shows lysates of human uterus tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human MRGPRF Monoclonal Antibody (Catalog # MAB8396) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for MRGPRF at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Flow Cytometry Detection of MRGPRF antibody in HEK293 Human Cell Line Transfected with Human MRGPRF and eGFP antibody by Flow Cytometry. View Larger

Detection of MRGPRF in HEK293 Human Cell Line Transfected with Human MRGPRF and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) human MRGPRF or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human MRGPRF Monoclonal Antibody (Catalog # MAB8396) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB002).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MRGPRF

MRGPRF (MAS-related GPR, Member F), also known as RTA, MRGF, GPR140 or GPR168, belongs to the G protein coupled receptor 1 family. MRGPRF may be upregulated during the process of CSF-1 or GM-CSF-induced human monocyte to macrophage differentiation (1).

References
  1. Hohenhaus D.M., et al. (2013) Immunobiology 218:1345.
Long Name
Mas-related G Protein-coupled Receptor Member F
Entrez Gene IDs
116535 (Human); 211577 (Mouse); 266762 (Rat)
Alternate Names
FLJ16111; FLJ29034; FLJ40998; FLJ53714; G protein-coupled receptor 140; G protein-coupled receptor 168; G protein-coupled receptor MrgF; GPR140; GPR168; G-protein coupled receptor 140; G-protein coupled receptor 168; mas-related G protein-coupled MRGF; Mas-related gene F protein; MAS-related GPR, member F; mas-related G-protein coupled receptor member F; MGC21621; mrgF; MRGFDKFZp586B2122; MRGPRF; RTA; seven transmembrane helix receptor

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Citation for Human MRGPRF Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Development and application of a high-content virion display human GPCR array
    Authors: Guan-Da Syu, Shih-Chin Wang, Guangzhong Ma, Shuang Liu, Donna Pearce, Atish Prakash et al.
    Nature Communications

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