Human MT2A Antibody

Name: Human MT2A Antibody
Brand: R&D Systems
SKU: MAB10176
Rating: 5 (1 reviews)

Catalog #: MAB10176 1 Review Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB10176-100
MAB10176-SP

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human MT2A Antibody Summary

Species Reactivity

Human

Specificity

Detects human MT2A in diect ELISAs.

Source

Monoclonal Mouse IgG_2A Clone # 1006811

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Synthetic peptide containing human MT2A
Accession # P02795

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunohistochemistry

5-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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MT2A in Human Liver. MT2A was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human MT2A Monoclonal Antibody (Catalog # MAB10176) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in hepatocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MT2A

Metallothionein-2 (MT2A) is a protein that is transcriptionally regulated by heavy metals and glucocorticoids. This Metallothionein (MT) binds zinc. MTs sequester and/or dispense zinc during stress and inflammation to protect cells against reactive oxygen species.

Long Name

Metallothionein 2A

Entrez Gene IDs

4502 (Human); 17750 (Mouse)

Alternate Names

MT2; MT-2; MT2A; MT-II