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Human Nanog PE-conjugated Antibody

Catalog #: IC1997P
5 Citations Datasheet / COA / SDS
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IC1997P
Detection of Nanog in BG01V Human Cells by Flow Cytometry.
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Citations (5)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Nanog PE-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human Nanog in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse Nanog is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human Nanog
Trp153-Val305
Accession # ABZ92376
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry Detection of Nanog antibody in BG01V Human Cells antibody by Flow Cytometry. View Larger

Detection of Nanog in BG01V Human Cells by Flow Cytometry. Three-day Endoderm-differentiated BG01V human embryonic stem cells (upper image) or untreated BG01V human embryonic stem cells (lower image) were stained with Goat Anti-Human Nanog PE-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC1997P, filled histogram) or isotype control antibody (Catalog # IC108P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Nanog

Nanog is a member of the homeobox family of DNA binding transcription factors that has been shown to maintain pluripotency of embryonic stem cells. Its expression is high in undifferentiated embryonic stem cells and is down-regulated during embryonic stem cell differentiation, concomitant with loss of pluripotency (1‑3).

References
  1. Mitsui, K. et al. (2003) Cell 11:631.
  2. Chambers, I. et al. (2003) Cell 113:643.
  3. Hart, A.H. et al. (2004) Dev. Dyn. 230:187.
Long Name
Nanog Homeobox
Entrez Gene IDs
79923 (Human); 71950 (Mouse)
Alternate Names
FLJ12581; FLJ40451; hNanog; homeobox protein NANOG; Homeobox transcription factor Nanog; homeobox transcription factor Nanog-delta 48; Nanog homeobox; Nanog

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Citations for Human Nanog PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Effect of ectopic high expression of transcription factor OCT4 on the "stemness" characteristics of human bone marrow-derived mesenchymal stromal cells
    Authors: X Guo, Y Tang, P Zhang, S Li, Y Chen, B Qian, H Shen, N Zhao
    Stem Cell Res Ther, 2019-06-03;10(1):160.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  2. The C-terminus of tissue factor pathway inhibitor alpha is required for its interaction with factors V and Va.
    J. Thromb. Haemost., 2012-09-01;10(9):1944-6.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Cross-presentation of tumour antigens by human induced pluripotent stem cell-derived CD141(+)XCR1+ dendritic cells.
    Gene Ther, 2011-11-10;19(10):1035-40.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Brief report: benchmarking human pluripotent stem cell markers during differentiation into the three germ layers unveils a striking heterogeneity: all markers are not equal.
    Stem Cells, 2011-09-01;29(9):1469-74.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Isolation and characterization of renal cancer stem cells from patient-derived xenografts.
    Authors: Hasmim Meriem, Bruno Stefania, Azzi Sandy et al.
    Oncotarget

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