Human Nanos2 Antibody

Catalog #: MAB2870 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB2870
MAB2870-SP

All Nanos2 Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Nanos2 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Nanos2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human Nanos1 is observed.

Source

Monoclonal Mouse IgG₁ Clone # 343255

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human Nanos2
Met1-Arg138
Accession # P60321

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human Nanos2

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Nanos2

Nanos2 is a cytoplasmic RNA-binding protein that belongs to the Nanos family of translational repressors. The 138 amino acid (aa) protein contains a 55 aa nanos‑type zinc finger domain with two C2HC motifs. Human and mouse Nanos2 share identity 75% aa sequence identity overall but 95% within the zinc finger RNA binding domain. In the mouse, Nanos2 is located in male germ cells and is essential for spermatogenesis.

Entrez Gene IDs

339345 (Human)

Alternate Names

nanos homolog 2 (Drosophila); nanos homolog 2; Nanos2; NOS2NOS-2