Human NEDD9/CASL Antibody

Catalog #: MAB5459 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB5459
MAB5459-SP

Detection of Human NEDD9/CASL by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human NEDD9/CASL Antibody Summary

Species Reactivity

Human

Specificity

Detects human NEDD9/CASL in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) NEDD4 or rhNEDD8 is observed.

Source

Monoclonal Mouse IgG_2A Clone # 584825

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human NEDD9/CASL
Lys153-Asn366
Accession # Q14511

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human NEDD9/CASL by Western Blot. Western blot shows lysates of Daudi human Burkitt's lymphoma cell line. PVDF Membrane was probed with 0.1 µg/mL of Mouse Anti-Human NEDD9/CASL Monoclonal Antibody (Catalog # MAB5459) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for NEDD9/CASL at approximately 110 kDa & 113 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NEDD9/CASL

NEDD9 (also HEF1 and CASL) is a 93 kDa (predicted) member of the CAS family of proteins. It is widely expressed, and serves to integrate integrin beta 1 and BCR signaling with cell mitotic pathways. Human NEDD9 is 834 amino acids (aa) in length. It contains one SH3 domain (aa 3-65), a Tyr-rich SH2-binding region (aa 90-350), one Ser-rich four alpha -helix bundle protein interaction site (aa 350‑650), and an HLH motif that mediates Cas protein dimerization (aa 710-760). NEDD9 usually runs at 105 or 115 kDa in SDS-PAGE due to phosphorylation. It is also cleaved near Leu361 to generate an active N-terminal 55 kDa fragment. One isoform shows a deletion of aa 155-188, while two others show 20 or 7 aa substitutions for aa 155-834 or 1-153, respectively. Over aa 153-366, human and mouse NEDD9 share 85% aa identity.

Long Name

Neural Precursor Cell Expressed Developmentally Down-Regulated Protein 9

Entrez Gene IDs

4739 (Human); 18003 (Mouse); 291044 (Rat)

Alternate Names

Cas scaffolding protein family member 2; CAS2; CASL; CAS-LCASS2cas-like docking; CASLdJ49G10.2; CRK-associated substrate-related protein; dJ49G10.2 (Enhancer of Filamentation 1 (HEF1)); dJ761I2.1 (enhancer of filamentation (HEF1)); dJ761I2.1; enhancer of filamentation 1; HEF1; HEF1Crk-associated substrate related; NEDD9; NEDD-9; Neural precursor cell expressed developmentally down-regulated protein 9; neural precursor cell expressed, developmentally down-regulated 9; p105; Renal carcinoma antigen NY-REN-12