Human Neprilysin-2/MMEL1 Antibody

Catalog #: AF2340
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF2340
AF2340-SP

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Citations (1)

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Neprilysin-2/MMEL1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Neprilysin-2/MMEL1 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross‑reactivity with recombinant human (rh) Neprilysin is observed and less than 1% cross-reactivity with rhECE-1 and rhECE-2 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Neprilysin-2/MMEL1
Gly69-Trp770
Accession # AAL08942

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human Neprilysin-2/MMEL1 (Catalog # 2340-ZN)

Flow Cytometry

2.5 µg/10⁶ cells

HEK293 cells

Immunoprecipitation

25 µg/mL

Conditioned cell culture medium spiked with Recombinant Human Neprilysin-2/MMEL1 (Catalog # 2340-ZN), see our available Western blot detection antibodies

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Neprilysin-2/MMEL1

Neprilysin-2 is a zinc protease of the neprilysin (NEP) family, which also includes NEP, ECE-2, PEX, DINE, Kell and several NEP-like proteins (1). It is encoded by the MMEL1 gene in the human genome and is also known as soluble secreted endopeptidase (SEP), Neprilysin-like 1 and Neprilysin-2 (2). Highly expressed in testis, the cDNA predicted a type II transmembrane protein with a short cytoplasmic tail and a large ectodomain. Both membrane-bound and soluble forms were comparable with regard to model substrates, pH optima and inhibitor profiles. The ectodomain of human Neprilysin-2/MMEL1 was expressed with an N-terminal His tag and purified.

References

Turner, A.J. et al. (2001) BioEssays 23:261.
Carpentier, M. et al. (2004) in Handbook of Proteolytic Enzymes. Barrett, A.J. et al. eds. p. 426.

Entrez Gene IDs

79258 (Human)

Alternate Names

EC 3.4.24; EC 3.4.24.11; MELL1; membrane metallo-endopeptidase-like 1; Membrane metallo-endopeptidase-like 2MGC119455; MGC119454; MMEL1; MMEL2; NEP2; NEP2(m); NEPIIMGC119456; Neprilysin II; Neprilysin2; Neprilysin-2; NL2; NL2NL1; SEP; soluble secreted endopeptidase; zinc metallopeptidase

Product Datasheets

Product Datasheet

COA

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Related Research Areas

Citation for Human Neprilysin-2/MMEL1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

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