Human Neurogenin-3 Antibody

Catalog #: MAB3444 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB3444
MAB3444-SP

Detection of Human Neurogenin‑3 by Western Blot.

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All Neurogenin-3 Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Neurogenin-3 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Neurogenin-3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human Neurogenin-1 is observed.

Source

Monoclonal Mouse IgG_2A Clone # 258602

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human Neurogenin-3
Met1-Leu214
Accession # Q9Y4Z2

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Neurogenin‑3 by Western Blot. Western blot shows lysates of human heart tissue, human placenta tissue, human prostate tissue, and human liver tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Neurogenin-3 Monoclonal Antibody (Catalog # MAB3444) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Neurogenin-3 at approximately 27 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Neurogenin-3

Neurogenin-3 (NGN3) is a 23 kDa developmentally expressed nuclear protein of the basic helix-loop-helix (bHLH) family, class B subfamily. bHLH proteins are transcription factors that form dimers to bind DNA. Neurogenin-3 expression is required for all endocrine lineage differentiation in the pancreas, including formation of islet beta cells. Neurogenin-3 is also neurogenic, controlling the number of dendrites developed by hippocampal neurons. Human Neurogenin-3 shows 76% and 77% amino acid identity with mouse and rat Neurogenin-3, respectively.

Entrez Gene IDs

50674 (Human); 11925 (Mouse); 60329 (Rat)

Alternate Names

Atoh5; BHLHA7; bHLHa7NGN-3; Class A basic helix-loop-helix protein 7; Math4B; NEUROG3; neurogenin 3; Neurogenin3; Neurogenin-3; NGN3; Protein atonal homolog 5; Relax