Human Neuropilin-1 Antibody

Catalog #: MAB38701
4 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB38701
MAB38701-SP

All Neuropilin-1 Antibodies

Product Details

Citations (4)

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Neuropilin-1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Neuropilin-1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody does not cross‑react with recombinant rat (rr) Neuropilin-1 or rhNeuropilin-2.

Source

Monoclonal Mouse IgG₁ Clone # 446915

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Neuropilin‑1
Phe22-Lys644
Accession # NP_001019799

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human Neuropilin‑1/BDCA4 (Catalog # 3870-N1)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Neuropilin-1

Neuropilin-1 (Npn-1, previously neuropilin; also known as CD304) is a 130-140 kDa type I transmembrane (TM) glycoprotein that regulates axon guidance and angiogenesis (1‑4). The full-length 923 amino acid (aa) human Npn-1 contains a 623 aa extracellular domain (ECD) that shows 92-95% aa identity with mouse, rat, bovine and canine Npn-1 (3, 4). The ECD contains two N-terminal CUB domains (termed a1a2), two domains with homology to coagulation factors V and VIII (b1b2) and a MAM (meprin) domain (c). C-terminally divergent splice variants with 704, 644, 609, and 551 aa lack the MAM and TM domains and are demonstrated or presumed to be soluble antagonists (1, 5-7). A 906 aa form lacks a TM segment, but secretion has not been found (8). The sema domains of Class III secreted semaphorins such as Sema3A bind Npn-1 a1a2 (9). Heparin, the heparin-binding forms of VEGF (VEGF₁₆₅, VEGF-B, and VEGF-E), PlGF (PlGF-2), and the C-terminus of Sema3 bind the b1b2 region (9, 10). Npn-1 and Npn-2 share 48% aa identity within the ECD and can form homo- and hetero-oligomers via interaction of their MAM domains (1). Neuropilins show partially overlapping expression in neuronal and endothelial cells during development (1, 2). Both neuropilins act as co-receptors with plexins, mainly plexin A3 and A4, to bind class III semaphorins that mediate axon repulsion (11). However, only Npn-1 binds Sema3A, and only Npn-2 binds Sema3F (1). Both are co-receptors with VEGF R2 (also called KDR or Flk-1) for VEGF₁₆₅ binding (1). Sema3A signaling can be blocked by VEGF₁₆₅, which has higher affinity for Npn-1 (12). Npn-1 is preferentially expressed in arteries during development or those undergoing remodeling (1, 2). Npn-1 is also expressed on dendritic cells and mediates DC-induced T cell proliferation (13).

References

Bielenberg, D.R. et al. (2006) Exp. Cell Res. 312:584.
Gu, C. et al. (2003) Dev. Cell 5:45.
He, Z. and M. Tessier-Lavigne (1997) Cell 90:739.
Soker, S. et al. (1998) Cell 92:735.
Gagnon, M.L. et al. (2000) Proc. Natl. Acad. Sci. USA 97:2573.
Cackowski, F.C. et al. (2004) Genomics 84:82.
Rossignol, M. et al. (2000) Genomics 70:211.
Tao, Q. et al. (2003) Angiogenesis 6:39.
Gu, C. et al. (2002) J. Biol. Chem. 277:18069.
Mamluk, R. et al. (2002) J. Biol. Chem. 277:24818.
Yaron, A. et al. (2005) Neuron 45:513.
Narazaki, M. and G. Tosato (2006) Blood 107:3892.
Tordjman, R. et al. (2002) Nat. Immunol. 3477.

Entrez Gene IDs

8829 (Human); 18186 (Mouse); 246331 (Rat); 100286859 (Porcine)

Alternate Names

BDCA4; BDCA-4; CD304 antigen; CD304; DKFZp686A03134; DKFZp781F1414; neuropilin 1; Neuropilin1; Neuropilin-1; NP1; NRP; NRP1; transmembrane receptor; Vascular endothelial cell growth factor 165 receptor; VEGF165R

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Related Research Areas

Citations for Human Neuropilin-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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Biomarker analysis of the phase II JO25567 study comparing erlotinib with or without bevacizumab in first-line advanced EGFR+ non-small-cell lung cancer
Authors: Makoto Nishio, Shinji Atagi, Koichi Goto, Yukio Hosomi, Takashi Seto, Toyoaki Hida et al.
Translational Lung Cancer Research
An Immune Atlas of Clear Cell Renal Cell Carcinoma
Authors: Stéphane Chevrier, Jacob Harrison Levine, Vito Riccardo Tomaso Zanotelli, Karina Silina, Daniel Schulz, Marina Bacac et al.
Cell
Biomarker results from the AVADO phase 3 trial of first-line bevacizumab plus docetaxel for HER2-negative metastatic breast cancer.
Authors: Miles D, de Haas S, Dirix L, Romieu G, Chan A, Pivot X, Tomczak P, Provencher L, Cortes J, Delmar P, Scherer S
Br J Cancer, 2013-02-19;108(5):1052-60.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P
Neuropilin-1 regulates platelet-derived growth factor receptor signalling in mesenchymal stem cells.
Authors: Ball SG, Bayley C, Shuttleworth CA, Kielty CM
Biochem. J., 2010-03-15;427(1):29-40.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot