Human Neuropilin-1 Quantikine ELISA Kit Summary
Product Summary
Precision
Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma, Urine, Human Milk
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 336 | 967 | 1890 | 329 | 934 | 1833 |
| Standard Deviation | 17.9 | 70.4 | 54.7 | 19.8 | 48.4 | 90.4 |
| CV% | 5.3 | 7.3 | 2.9 | 6 | 5.2 | 4.9 |
Recovery
The recovery of human Neuropilin-1 spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 91 | 86-99 |
| Cell Lysates (n=3) | 101 | 94-108 |
| Urine (n=4) | 99 | 85-117 |
Linearity
Scientific Data
Product Datasheets
Preparation and Storage
Background: Neuropilin-1
Neuropilin-1 (Npn-1, previously known as neuropilin) and Neuropilin-2 (previously known as Npn-1-related molecule) are type I transmembrane proteins that bind distinct members of the class III secreted semaphorin subfamily that are implicated in repulsive axon guidance. Neuropilin extracellular domains contain two CUB (complement-binding) domains, two domains with homology to coagulation factors V and VIII, and a MAM (meprin) domain. In the absence of ligands, neuropilins can form homo- and hetero-oligomers via their MAM domains. Expression in developing neurons of the central and peripheral nervous systems is somewhat overlapping but distinct. Npn-1 and Npn-2 are also receptors for VEGF165 on endothelial and tumor cells.
Assay Procedure
Refer to the product- Prepare all reagents, standard dilutions, and samples as directed in the product insert.
- Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
- Add 50 µL of Assay Diluent to each well.
- Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
- Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
- Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
- Aspirate and wash 4 times.
- Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.
- Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
Citations for Human Neuropilin-1 Quantikine ELISA Kit
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
3
Citations: Showing 1 - 3
Filter your results:
Filter by:
-
Serum Concentration of Selected Angiogenesis-Related Molecules Differs among Molecular Subtypes, Body Mass Index and Menopausal Status in Breast Cancer Patients
Authors: D Balalis, D Tsakogiann, E Kalogera, S Kokkali, E Tripodaki, A Ardavanis, D Manatakis, D Dimas, N Koufopoulo, F Fostira, D Korkolis, I Misitzis, N Vassos, C Spiliopoul, D Vlachodimi, G Bletsa, N Arkadopoul
Oncogene, 2022-07-14;11(14):.
Species: Human
Sample Types: Serum
-
ANGPTL4 influences the therapeutic response of neovascular age-related macular degeneration patients by promoting choroidal neovascularization
Authors: Y Qin, A Dinabandhu, X Cao, JC Sanchez, K Jee, M Rodrigues, C Guo, J Zhang, J Vancel, D Menon, NS Khan, T Ma, SY Tzeng, YJ Daoud, JJ Green, GL Semenza, S Montaner, A Sodhi
JCI Insight, 2022-07-08;0(0):.
Species: Human
Sample Types: Aqueous Humor
-
Neovascularization Potential of Blood Outgrowth Endothelial Cells From Patients With Stable Ischemic Heart Failure Is Preserved
Authors: D Dauwe, B Pelacho, A Wibowo, AS Walravens, K Verdonck, H Gillijns, E Caluwe, P Pokreisz, N van Gastel, G Carmeliet, M Depypere, F Maes, N Vanden Dri, W Droogne, J Van Cleemp, J Vanhaecke, F Prosper, C Verfaillie, A Luttun, S Janssens
J Am Heart Assoc, 2016-04-18;5(4):.
Species: Human
Sample Types: Cell Culture Supernates
FAQs
No product specific FAQs exist for this product, however you may
View all ELISA FAQsReviews for Human Neuropilin-1 Quantikine ELISA Kit
Average Rating: 5 (Based on 1 Review)
Have you used Human Neuropilin-1 Quantikine ELISA Kit?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
Filter by:
Measured plasma at 200-fold dilution ad measured urine undiluted. We were able to obtain values in range in both biofluids.
