Human Neurturin Antibody

Catalog #: MAB3871 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB3871
MAB3871-SP

All Neurturin Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Neurturin Antibody Summary

Species Reactivity

Human

Specificity

Detects human Neurturin in direct ELISAs and Western blots. In sandwich immunoassays, no cross-reactivity with recombinant mouse Neurturin, recombinant human (rh) TGF-alpha, rhTGF-beta 2, rhTGF-beta 1.2, rhTGF-beta 3, rhGDNF, rhLAP, or recombinant rat TGF-beta 5 is observed.

Source

Monoclonal Mouse IgG₁ Clone # 126424

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human Neurturin
Ala96-Val197
Accession # Q99748

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human Neurturin (Catalog # 1297-NE)

ELISA

This antibody functions as an ELISA capture antibody when paired with Mouse Anti-Human Neurturin Monoclonal Antibody (Catalog # MAB3872).

This product is intended for assay development on various assay platforms requiring antibody pairs.

Blockade of Receptor-ligand Interaction

In a functional ELISA, 0.06-0.3 µg/mL of this antibody will block 50% of the binding of 50 ng/mL of Recombinant Human Neurturin (Catalog # 1297-NE) to immobilized Recombinant Human GFR alpha -2 Fc Chimera (Catalog # 613‑FR) coated at 1 µg/mL (100 µL/well). At 5 μg/mL, this antibody will block >90% of the binding.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Neurturin

Neurturin is a member of the GDNF family of ligands and is distantly related to TGF-beta family ligands. It is a disulfide-linked homodimer that interacts with a receptor complex composed of a high affinity ligand binding component (GFR alpha 1 or GFR alpha 2) and a common signaling component, cRET. Neurturin promotes the survival of a variety of neurons and possibly other cell types.

Entrez Gene IDs

4902 (Human); 18188 (Mouse)

Alternate Names

Neurturin; NRTN; NTN