Human NF-H Antibody

Catalog #: AF3108
3 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF3108
AF3108-SP

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All NF-H Antibodies

Product Details

Citations (3)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human NF-H Antibody Summary

Species Reactivity

Human

Specificity

Detects human NF-H in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross-reactivity with recombinant human (rh) NF-M and rhNF-L is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human NF-H
Met1-Ala380
Accession # P12036

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Human NF-H

Simple Western

2.5 µg/mL

Human cerebellum

Immunohistochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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NF-H in Human Brain. NF-H was detected in immersion fixed paraffin-embedded sections of human brain (cerebellum) using 15 µg/mL Goat Anti-Human NF-H Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3108) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Simple Western

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Detection of Human NF‑H by Simple Western^TM. Simple Western shows lysates of human cerebellum, loaded at 0.2 mg/ml. A specific band was detected for NF‑H at approximately 373 kDa (as indicated) using 2.5 µg/mL of Goat Anti-Human NF‑H Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3108). This experiment was conducted under reducing conditions and using the 66-440kDa separation system.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NF-H

Neurofilament (NF)-H is a 200 kDa neurofilament protein and is one of three NF subunits. In the protein region used for immunization, human NF-H shares approximately 92% sequence identity with the mouse or rat protein.

Long Name

Neurofilament Protein, Heavy Chain

Entrez Gene IDs

4744 (Human)

Alternate Names

200 kDa neurofilament protein; KIAA0845; NEFH; Neurofilament Heavy (200kDa); neurofilament heavy polypeptide; Neurofilament triplet H protein; neurofilament, heavy polypeptide 200kDa; neurofilament, heavy polypeptide; NF200; NFH; NF-H

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human NF-H Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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