Human NF-M Antibody

Catalog #: MAB3029 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB3029
MAB3029-SP

Western Blot Shows Human NF‑M Specificity by Using Knockout Cell Line.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human NF-M Antibody Summary

Species Reactivity

Human

Specificity

Detects human NF-M in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) NF-H or rhNF-L is observed.

Source

Monoclonal Mouse IgG₁ Clone # 327921

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human NF-M
Ser2-Ser358
Accession # P07197

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

NF‑M is specifically detected in HeLa human cervical epithelial carcinoma parental cell line but is not detectable in NF‑M knockout HeLa cell line.

Knockout Validated

NF‑M is specifically detected in HeLa human cervical epithelial carcinoma parental cell line but is not detectable in NF‑M knockout HeLa cell line.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Knockout Validated

Western Blot Shows Human NF-M Antibody Specificity by Using Knockout Cell Line.

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Western Blot Shows Human NF‑M Specificity by Using Knockout Cell Line. Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and NF-M knockout HeLa cell line (KO). PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human NF-M Monoclonal Antibody (Catalog # MAB3029) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for NF-M at approximately 155 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NF-M

Human NF-M (neurofilament-medium length) is a 145‑150 kDa phosphorylated glycoprotein that belongs to the type IV intermediate filament family. It is 915 amino acids in length and contains a 100 amino acid (aa) N-terminal globular region, a 320 aa alpha -helical rod, or central region, and a 500 aa globular tail. The rod region contains a series of heptad repeats that contribute to a coiled-coil interaction with an adjacent NF-M molecule, the first step in intermediate filament oligomerization. The C-terminus shows phosphorylation and O-linked glycosylation. Human NF-M aa 2‑358, which includes most of the rod region, shares ~98% aa sequence identity with corresponding region of mouse, rat and canine NF-M.

Long Name

Neurofilament Protein, Medium Chain

Entrez Gene IDs

4741 (Human)

Alternate Names

NEF3; NEF3neurofilament medium polypeptide; NEFM; Neurofilament 3; neurofilament, medium polypeptide 150kDa; neurofilament, medium polypeptide; neurofilament-3 (150 kD medium); NFM; NF-M; NF-M160 kDa neurofilament protein; NFMNeurofilament triplet M protein