Human NF2/Merlin Antibody

Name: Human NF2/Merlin Antibody
Brand: R&D Systems
SKU: MAB5616
Rating: 4 (1 reviews)

Catalog #: MAB5616 1 Review Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB5616
MAB5616-SP

Detection of Human NF2/Merlin by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human NF2/Merlin Antibody Summary

Species Reactivity

Human

Specificity

Detects human NF2/Merlin in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human NF1 is observed.

Source

Monoclonal Mouse IgG_2B Clone # 534428

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human NF2/Merlin
Met363-Lys578
Accession # P35240

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human NF2/Merlin by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and human peripheral blood mononuclear cells (PBMC). PVDF Membrane was probed with 2 µg/mL of Mouse Anti-Human NF2/Merlin Monoclonal Antibody (Catalog # MAB5616) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for NF2/Merlin at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NF2/Merlin

NF2 (Neurofibromin-2; also Merlin) is a 70‑75 kDa tumor suppressor protein belonging to the ERM (ezrin, radixin, moesin) family of proteins. It participates in contact‑mediated cell adhesion, thereby blocking cell proliferation and migration. Human NF2 is 595 amino acids (aa) in length. It contains three general regions. There is an N‑terminal FERM domain (aa 1‑302), an alpha -helical rod central region (aa 303‑478), and a unique carboxy-terminal domain (aa 479‑595). Confluence requires an absence of phosphorylation at Ser516 and Ser10. There are multiple potential splice variants. Four short forms less than 260 aa in length lack the aa range used for antigen immunization. There are at least eight additional isoforms that basically show an alternate start site coupled to an 11 aa substitution for aa 579‑595. Lack of NF2 is associated with neurofibromatosis type II.

Long Name

Neurofibromin 2

Entrez Gene IDs

4771 (Human); 18016 (Mouse); 25744 (Rat)

Alternate Names

ACN; BANF; Merlin; moesin-ezrin-radixin like; Moesin-ezrin-radixin-like protein; moesin-ezrin-radizin-like protein; neurofibromin 2 (bilateral acoustic neuroma); neurofibromin 2 (merlin); neurofibromin-2; NF2; SCH; Schwannomerlin; Schwannomin