Human NFATC3 Antibody

Catalog #: AF5834
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF5834
AF5834-SP

Detection of Human NFATC3 by Western Blot.

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All NFATC3 Antibodies

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Citations (1)

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Human NFATC3 Antibody Summary

Species Reactivity

Human

Specificity

Detects human NFATC3 in Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human NFATC3
Asp900-Asp1035
Accession # Q12968

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

293T human embryonic kidney cell line, HeLa human cervical epithelial carcinoma cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human NFATC3 by Western Blot. Western blot shows lysates of 293T human embryonic kidney cell line and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Human NFATC3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5834) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for NFATC3 at approximately 145 - 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NFATC3

NFATC3 (Nuclear factor of activated T cells C3; also NFAT4 and NFATx) is a 145‑150 kDa member of the NFAT family of transcription factors. NFATC3 is widely expressed being found in DP thymocytes, CD4+ T cells, smooth muscle cells, Schwann cells and mast cells. NFATC3 regulates gene transcription as part of a NFATC transcription complex. It is normally cytoplasmic and phosphorylated. Upon a rise in intracellular Ca⁺², dephosphorylation occurs via calcineurin, and NFATC3 enters the nucleus. Human NFATC3 is 1075 amino acids (aa) in length. It contains a calcineurin binding site (aa 109‑114), two nuclear localization sites (aa 273‑275 and 686‑688), one nuclear export site (aa 1032‑1041) and an RH domain that binds DNA (aa 433‑592). There are multiple isoforms with different tissue expression profiles. Over aa 900‑1035, human NFATC3 shares 93% aa identity with mouse NFATC3.

Long Name

Nuclear Factor of Activated T Cells C3

Entrez Gene IDs

4775 (Human); 18021 (Mouse)

Alternate Names

NFAT4; NF-AT4; NFAT4nuclear factor of activated T-cells, cytoplasmic 3; NFATC3; NF-ATc3; NFATX; nuclear factor of activated T-cells c3 isoform IE-Xa; nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 3; T cell transcription factor NFAT4; T-cell transcription factor NFAT4

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Citation for Human NFATC3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

A Peptidyl Inhibitor that Blocks Calcineurin–NFAT Interaction and Prevents Acute Lung Injury
Authors: Patrick G. Dougherty, Manjula Karpurapu, Amritendu Koley, Jessica K. Lukowski, Ziqing Qian, Teja Srinivas Srinivas Nirujogi et al.
Journal of Medicinal Chemistry