Human NGL-3/LRRC4B Antibody

Catalog #: MAB69191
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB69191
MAB69191-SP

Detection of Human NGL‑3/LRRC4B by Western Blot.

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All NGL-3/LRRC4B Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human NGL-3/LRRC4B Antibody Summary

Species Reactivity

Human

Specificity

Detects human NGL-3/LRRC4B in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) LRRC3, rhLRRC4, or rhLRRC32 is observed.

Source

Monoclonal Mouse IgG_2A Clone # 702008

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human NGL-3/LRRC4B
Ala47-Lys576
Accession # Q9NT99

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human NGL‑3/LRRC4B by Western Blot. Western blot shows lysates of human brain (hypothalamus) tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human NGL-3/LRRC4B Monoclonal Antibody (Catalog # MAB69191) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for NGL-3/LRRC4B at approximately 115 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NGL-3/LRRC4B

NGL-3 (netrin-G ligand-3), also called LRRC4B (Leu-rich repeat/LRR-containing glycoprotein 4B), is a 72 kDa (predicted) post-synaptic adhesion molecule of the LRR protein family. Mature human LRRC4B is a 678 amino acid (aa) type I transmembrane protein with a 541 aa extracellular region (aa 36-576) containing nine LRRs, a C2-type Ig-like domain, and a Thr-rich segment. Over the region used as an immunogen, human LRRC4B shares 97% aa sequence identity with mouse and rat LRRC4B. LRRC4B also shares ~55% aa identity with LRRC4C/NGL-1 and LRRC4/NGL-2, but each recognizes different ligands. LRRC4B recognition of the receptor tyrosine phosphatases LAR/PTPRF, PTPR-d and PTPR-s induces excitatory synapse formation.

Long Name

Netrin-G3 Ligand/Leucine-rich Repeat Containing 4B

Entrez Gene IDs

94030 (Human); 272381 (Mouse); 308571 (Rat)

Alternate Names

DKFZp761A179; HSM; Leucine Rich Repeat Containing 4B; Leucine-Rich Repeat-Containing Protein 4B; Leucine-Rich Repeats And Immunoglobulin-Like Domains 4; LRIG4; LRRC4B; Netrin-G3 Ligand; NGL3; NGL-3

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Netrins and Receptors

Citation for Human NGL-3/LRRC4B Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1