Human NgR3/NgRH2 Antibody

Catalog #: MAB2920 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB2920
MAB2920-SP

All NgR3/NgRH2 Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human NgR3/NgRH2 Antibody Summary

Species Reactivity

Human

Specificity

Detects human NgR3/NgRH2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human NgRH1 is observed.

Source

Monoclonal Mouse IgG₁ Clone # 319123

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human NgR3/NgRH2
Cys25-Ala419
Accession # Q86UN2

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human NgR3/NgRH2

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NgR3/NgRH2

NgR3, a Nogo-66 receptor homolog alternately known as reticulon 4 receptor-like 1, Nogo receptor homolog 2 (NgRH2), or NgR3, is an approximately 60 kDa glycosylphosphatidylinositol-anchored glycoprotein expressed primarily in the brain. It contains eight leucine-rich repeats (LRR) flanked by conserved cysteines. Although LRR are typically involved in protein-protein interactions, NgR3 has not been shown to bind known Nogo-66 receptor ligands. Mature human NgR3 shares 88%, 88%, 48% and 44% amino acid identity with mature mouse NgR3, rat NgR3, human NgRH1 and human NgR, respectively.

Long Name

Nogo-66 Receptor-related Protein 3/Nogo-66 Receptor Homolog 2/Reticulon-4 Receptor-like 1

Entrez Gene IDs

146760 (Human); 237847 (Mouse)

Alternate Names

DKFZp547J144; NgR3; NgRH2; NGRL2; nogo receptor-like 2; Nogo-66 receptor homolog 2; Nogo-66 receptor-related protein 3; reticulon 4 receptor-like 1; reticulon-4 receptor-like 1; RTN4RL1