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Human NKG2C/CD159c Antibody

Catalog #: MAB138
22 Citations 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB138-100
MAB138-SP
Detection of Human NKG2C/CD159c/KLRC2 by Flow Cytometry
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Product Details
Citations (22)
FAQs
Supplemental Products
Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human NKG2C/CD159c Antibody Summary

Species Reactivity
Human
Specificity
Detects human NKG2C/CD159c heterodimer with CD94 in flow cytometry. It does not cross-react with the human NKG2A/CD94 heterodimer or with the human CD94 homodimer.
Source
Monoclonal Mouse IgG1 Clone # 134591
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
BaF3 mouse pro-B cell line transfected with human NKG2C/CD159c and CD94
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
2.5 µg/106 cells
Human whole blood CD56+ natural killer cells
CyTOF-reported
Horowitz, A. et al. (2015) J. Immunol. 195: 4524. Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of Human NKG2C/CD159c/KLRC2 by Flow Cytometry View Larger

Detection of Human NKG2C/CD159c/KLRC2 by Flow Cytometry Phenotypic characterization of NK cells from HIV-infected individuals with different levels of adaptation to HCMV infection. To identify the NK cell population, (A) PBMC were selected and dead cells excluded. NK cells were identified by expression of CD56 in the absence of CD3 and further characterized for percentage expressing NKG2C, CD57, Tim-3, and FcR gamma in a representative plot. Summary plots depict the percentage of total NK cells that are positive for (B) NKG2C or (C) FcR gamma. Individual donors were grouped based on HCMV serostatus (HCMVneg) and fraction of NK cells expressing NKG2C within the HCMVpos donors (NKG2Clo or NKG2Chi). Correlation between NK cell NKG2C and FcR gamma expression was assessed in (D) with Spearman correlation coefficient (r) calculated and the probability of a significant correlation (p) shown on the graph. Error bars in (B) for the HCMVneg group represent median with IQR, while all others represent mean ± SD. Groups were compared using Mann–Whitney U-tests. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30483249), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NKG2C/CD159c

Human NKG2C (NK cell Group 2 isoform C; Killer cell lectin-like receptor subfamily C, member 2) is a member of the C-type lectin-like superfamily of proteins. Natural killer (NK) receptors are expressed in both NK cells and cytotoxic CD8+ T cells and have both activating and inhibitory members (1-3). Regulation of the balance between the activating and inhibitory receptors is important and lack of such regulation has been implicated in autoimmunity (4). The NKG2 family includes seven receptors: NKG2A, -B, -C, -D, -E, -F, and -H, which is the longer isoform of NKG2E. Except for NKG2D and NKG2F, the NKG2 family members form heterodimers with CD94 (5, 6). NKG2C interacts with the adapter molecule DAP12 and acts as activating receptor when heterodimerized with CD94 (7). Human NKG2C is synthesized as a 231 amino acid (aa) protein that includes a 70 aa cytoplasmic domain, a 23 aa transmembrane segment, and a 138 aa extracellular domain (ECD). Within the ECD, human NKG2C shares 40% sequence identity with mouse NKG2C. NKG2C-CD94 heterodimers bind to the widely expressed nonclassical MHC-I molecule, HLA-E (Qa-1b in mouse), which presents a peptide derived from the signal peptide of classical MHC-I molecules (8, 9). Triggering the NKG2C-CD94 complex may activate the cytolytic activity and cytokine production of NK and CD8+ T cells (8, 10). Human cytomegalovirus (HCMV) infection promotes the differentiation and expansion of NKG2C+ NK cell subsets, possibly involving a cognate interaction of CD94/NKG2C with ligand(s) displayed by HCMV‑infected cells (11, 12).

References
  1. Orbelyan, G.A. et al. (2014) J. Immunol. 193:610.
  2. Tassi I. et al. (2006) Immnunol Rev. 214:92.
  3. Lanier, L.L. (2008) Nat. Immunol. 9:495.
  4. Schleinitz, N. et al. (2010) Immunology. 174:2878.
  5. Lopez-Botet, M. et al. (2000) Hum. Immunol. 61:7.
  6. Braud, V.M. et al. (1998) Nature. 391:795.
  7. Lanier, L.L. (1998) Immunity 8:693.
  8. Vance, R.E. et al. (1999) J Exp Med 190:1801.
  9. Kaiser B.K. et al. (2005) J Immunol 174:2878.
  10. Bellón T. et al. (1999) J Immunol 162:3996.
  11. Pupuleku A. et al. (2017) Front Immunol. 8:1317.
  12. Hammer Q. et al. (2018) Nat Immunol. 19:453.
Long Name
Natural Killer G2C
Entrez Gene IDs
3822 (Human); 16642 (Mouse)
Alternate Names
CD159 antigen-like family member C; CD159c antigen; CD159c; killer cell lectin-like receptor subfamily C, member 2; KLRC2; MGC138244; NK cell receptor C; NKG2-C type II integral membrane protein; NKG2C; NKG2-C; NKG2CNKG2-C-activating NK receptor

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Citations for Human NKG2C/CD159c Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

22 Citations: Showing 1 - 10
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  1. Evaluation of the In Vitro Capacity of Anti-Human Cytomegalovirus Antibodies to Initiate Antibody-Dependent Cell Cytotoxicity
    Authors: d'Angelo, P;Zavaglio, F;Gabanti, E;Zelini, P;Fornara, C;Bernuzzi, S;Spinillo, A;Lilleri, D;Baldanti, F;
    Microorganisms
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. NK Cell Subset Redistribution and Antibody Dependent Activation after Ebola Vaccination in Africans
    Authors: HR Wagstaffe, O Anzala, H Kibuuka, Z Anywaine, SB Sirima, R Thiébaut, L Richert, Y Levy, C Lacabaratz, V Bockstal, K Luhn, M Douoguih, MR Goodier
    Vaccines, 2022-05-31;10(6):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Extracellular Vesicle-Mediated Secretion of HLA-E by Trophoblasts Maintains Pregnancy by Regulating the Metabolism of Decidual NK Cells
    Authors: L Jiang, H Fei, X Jin, X Liu, C Yang, C Li, J Chen, A Yang, J Zhu, H Wang, X Fei, S Zhang
    International journal of biological sciences, 2021-10-22;17(15):4377-4395.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Deconvoluting global cytokine signaling networks in natural killer cells
    Authors: GM Wiedemann, EK Santosa, S Grassmann, S Sheppard, JB Le Luduec, NM Adams, C Dang, KC Hsu, JC Sun, CM Lau
    Nature Immunology, 2021-04-15;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Expansions of adaptive-like NK cells with a tissue-resident phenotype in human lung and blood
    Authors: D Brownlie, M Scharenber, JE Mold, J Hård, E Kekäläinen, M Buggert, S Nguyen, JN Wilson, M Al-Ameri, HG Ljunggren, N Marquardt, J Michaëlsso
    Proceedings of the National Academy of Sciences of the United States of America, 2021-03-16;118(11):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Posttransplant Cyclophosphamide and Antithymocyte Globulin versus Posttransplant Cyclophosphamide as Graft-versus-Host Disease Prophylaxis for Peripheral Blood Stem Cell Haploidentical Transplants: Comparison of T Cell and NK Effector Reconstitution
    Authors: DR Makanga, T Guillaume, C Willem, N Legrand, K Gagne, A Cesbron, K Gendzekhad, P Peterlin, A Garnier, A Le Bourgeo, MC Béné, P Chevallier, C Retière
    J. Immunol., 2020-08-03;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. Cytomegalovirus-Driven Adaption of Natural Killer Cells in NKG2Cnull Human Immunodeficiency Virus-Infected Individuals
    Authors: EM Comeau, KA Holder, NJ Fudge, MD Grant
    Viruses, 2019-03-09;11(3):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. CD56-negative NK cells with impaired effector function expand in CMV and EBV co-infected healthy donors with age
    Authors: B Müller-Dur, J Grählert, OP Devine, AN Akbar, C Hess
    Aging (Albany NY), 2019-01-27;11(2):724-740.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. Proliferative capacity exhibited by human liver-resident CD49a+CD25+ NK cells
    Authors: G Martrus, T Kautz, S Lunemann, L Richert, L Glau, W Salzberger, H Goebels, A Langenecke, L Hess, T Poch, C Schramm, KJ Oldhafer, M Koch, E Tolosa, B Nashan, M Altfeld
    PLoS ONE, 2017-08-09;12(8):e0182532.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  10. Severe symptomatic primary HCMV infection despite effective innate and adaptive immune responses
    Authors: Raphaëlle Riou
    J. Virol, 2017-02-14;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  11. Ascorbic acid promotes proliferation of natural killer cell populations in culture systems applicable for natural killer cell therapy.
    Authors: Huijskens M, Walczak M, Sarkar S, Atrafi F, Senden-Gijsbers B, Tilanus M, Bos G, Wieten L, Germeraad W
    Cytotherapy, 2015-03-05;17(5):613-20.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  12. Cutting edge: identification and characterization of human intrahepatic CD49a+ NK cells.
    Authors: Marquardt N, Beziat V, Nystrom S, Hengst J, Ivarsson M, Kekalainen E, Johansson H, Mjosberg J, Westgren M, Lankisch T, Wedemeyer H, Ellis E, Ljunggren H, Michaelsson J, Bjorkstrom N
    J Immunol, 2015-02-11;194(6):2467-71.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  13. MicroRNA editing facilitates immune elimination of HCMV infected cells.
    Authors: Nachmani D, Zimmermann A, Oiknine Djian E, Weisblum Y, Livneh Y, Khanh Le V, Galun E, Horejsi V, Isakov O, Shomron N, Wolf D, Hengel H, Mandelboim O
    PLoS Pathog, 2014-02-27;10(2):e1003963.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  14. Epstein-Barr virus coinfection in children boosts cytomegalovirus-induced differentiation of natural killer cells.
    Authors: Saghafian-Hedengren S, Sohlberg E, Theorell J, Carvalho-Queiroz C, Nagy N, Persson J, Nilsson C, Bryceson Y, Sverremark-Ekstrom E
    J Virol, 2013-10-02;87(24):13446-55.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  15. Modulation of the natural killer cell KIR repertoire by cytomegalovirus infection.
    Authors: Charoudeh H, Terszowski G, Czaja K, Gonzalez A, Schmitter K, Stern M
    Eur J Immunol, 2012-12-12;43(2):480-7.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  16. Human Herpesvirus 8 (HHV8) sequentially shapes the NK cell repertoire during the course of asymptomatic infection and Kaposi sarcoma.
    Authors: Dupuy S, Lambert M, Zucman D, Choukem SP, Tognarelli S, Pages C, Lebbe C, Caillat-Zucman S
    PLoS Pathog., 2012-01-12;8(1):e1002486.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  17. NKG2A inhibits NKG2C effector functions of gammadelta T cells: implications in health and disease.
    Authors: Angelini DF, Zambello R, Galandrini R, Diamantini A, Placido R, Micucci F, Poccia F, Semenzato G, Borsellino G, Santoni A, Battistini L
    J. Leukoc. Biol., 2010-10-15;89(1):75-84.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, Functional Assay
  18. NK cell terminal differentiation: correlated stepwise decrease of NKG2A and acquisition of KIRs.
    Authors: Beziat V, Descours B, Parizot C, Debre P, Vieillard V
    PLoS ONE, 2010-08-06;5(8):e11966.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  19. CD94 surface density identifies a functional intermediary between the CD56bright and CD56dim human NK-cell subsets.
    Authors: Yu J, Mao HC, Wei M, Hughes T, Zhang J, Park IK, Liu S, McClory S, Marcucci G, Trotta R, Caligiuri MA
    Blood, 2009-11-06;115(2):274-81.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  20. Fully functional NK cells after unrelated cord blood transplantation.
    Authors: Beziat V, Nguyen S, Lapusan S, Hervier B, Dhedin N, Bories D, Uzunov M, Boudifa A, Trebeden-Negre H, Norol F, Marjanovic Z, Marie JP, Vernant JP, Debre P, Rio B, Vieillard V
    Leukemia, 2009-01-08;23(4):721-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  21. A novel subset of NK cells expressing high levels of inhibitory FcgammaRIIB modulating antibody-dependent function.
    Authors: Dutertre CA, Bonnin-Gelize E, Pulford K, Bourel D, Fridman WH, Teillaud JL
    J. Leukoc. Biol., 2008-08-21;84(6):1511-20.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  22. Activating and inhibitory receptors on synovial fluid natural killer cells of arthritis patients: role of CD94/NKG2A in control of cytokine secretion.
    Authors: de Matos CT, Berg L, Michaelsson J, Fellander-Tsai L, Karre K, Soderstrom K
    Immunology, 2007-05-22;122(2):291-301.
    Species: Human
    Sample Types: Synovial Fluid
    Applications: Flow Cytometry

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Human NKG2C/CD159c Antibody
By Stephanie Weiland on 02/22/2016
Application: ELISA Sample Tested: AML-193 human acute monocytic leukemia cell line Species: B. subtilis