Human NKG2C/CD159c APC-conjugated Antibody

Detection of NKG2C/CD159c in Human PBMC lymphocytes by Flow Cytometry. Human peripheral blood mononuclear cell (PBMC) lymphocytes were stained with Mouse Anti-Human NCAM-1/CD56 PE-conjugated Monoclonal Antibody (Catalog # FAB2408P) and either (A) Mouse Anti-Human NKG2C/CD159c APC-conjugated Monoclonal Antibody (Catalog # FAB138A) or (B) Mouse IgG₁Allophycocyanin Isotype Control (Catalog # IC002A). View our protocol for Staining Membrane-associated Proteins.

Detection of Human NKG2C/CD159c/KLRC2 by Flow Cytometry Cell surface expression of NKG2C and gene expression of CD57+NKG2C+ NK cells in healthy subjects and HIV-1 chronically infected individuals. a Flow cytometric gating strategy of sorted CD57+NKG2C+ NK cells. b Graphical analysis of frequency of CD57+NKG2C+ NK cells in healthy (black) and HIV-1-infected individuals (red). Mann–Whitney test was used to determine significance between frequencies of each group. c Volcano plot displaying upregulated (red), downregulated (blue), and stably expressed (gray) pre-selected genes, involved in NK cell-mediated responses to HIV-1 infection. Size of each data point is calculated as −log10(p-value) × log2(FC), p-value cutoff p < 0.05, fold change cutoff > 2 or <1/2. Transcriptional analysis from six healthy subjects and five HIV-1 chronically infected individuals from one independent experiment is represented Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/s41467-018-03618-w), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Human NKG2C/CD159c/KLRC2 by Flow Cytometry Cell surface expression of NKG2C and gene expression of CD57+NKG2C+ NK cells in vaccinated individuals. a Flow cytometric gating strategy of sorted CD57+NKG2C+ NK cells. b Graphical analysis of frequency of CD57+NKG2C+ NK cells in vaccinated individual’s pre (blue)- and post (red) vaccination. Wilcoxon matched-pair sign-rank test was used to determine significance between frequencies of each group. c Volcano plot displaying upregulated (red) and stably expressed (gray) pre-selected genes, involved in NK cell-mediated responses to MVA vaccination. Size of each data point is calculated as −log10(p-value) × log2(FC), p-value cutoff p < 0.05, fold change cutoff > 2 or <1/2. Transcriptional analysis from 11 vaccinated individuals and 1 independent experiment is represented Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/s41467-018-03618-w), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Human NKG2C/CD159c/KLRC2 by Flow Cytometry Distribution of CD57+NKG2C+ NK frequencies in different groups. Flow cytometry was done on PBMC to assess CD57+NKG2C+ NK frequency by gating on lymphocytes, excluding CD3+ cells, gating on CD56dim cells, and plotting NKG2C versus CD57 expression. Horizontal lines bisecting groups represent medians with IQR shown above and below them. Significant differences between medians are shown above lines spanning the groups compared. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/27314055), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Human NKG2C/CD159c/KLRC2 by Flow Cytometry Frequency of responding NK cells across multiple stimulation conditions. a Top row: overall successive gating strategy demonstrates initial broad gating on forward and side scatter to include large lymphocytes. Forward area and height are used to discriminate single cells followed by identification of viable cells. Monocytes and B cells are excluded using CD14 and CD19, and T cells are excluded using CD3 and CD4. Bottom row: CD56 and CD16 are used to identify NK cells and changes in CD16 expression across stimulation conditions. Black gate is the total NK population and red gate is CD56 dim population. b A univariate analysis of the frequency of CD107a, INF-gamma, TNF, granzyme B, perforin, and eomesodermin expression from the total NK cell population as well as CD57 and CD8 expression are shown. c Pie charts of classic Boolean analysis of five functional markers measured (CD107a, IFN-gamma, TNF, granzyme B, and perforin). Comparison of pies and nonparametric partial permutation tests (Monte Carlo simulation) are performed to determine statistical significance between pies (p-value < 0.0001)69. d Graph of classic Boolean analysis of five functional markers measured. Four healthy subjects from four independent experiments in each stimulation condition are included. CD107a, IFN-gamma, and TNF are mock subtracted (i.e., stimulated condition − unstimulated result). Black bars represent the standard error mean (SEM) for each stimulation condition calculated as the variance of the sampling distribution obtained, equal to the variance of the population divided by the sample size Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/s41467-018-03618-w), licensed under a CC-BY license. Not internally tested by R&D Systems.