Human NKp80/KLRF1 Antibody

Catalog #: MAB1900
2 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB1900
MAB1900-SP

All NKp80/KLRF1 Antibodies

Product Details

Citations (2)

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human NKp80/KLRF1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human NKp80/KLRF1 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) NKp30, rhNKp44, rhNKp46, or recombinant mouse NKp46 is observed.

Source

Monoclonal Rat IgG_2A Clone # 239127

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human NKp80/KLRF1
Val66-Tyr231
Accession # Q9NZS2

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Flow Cytometry

2.5 µg/10⁶ cells

Human whole blood CD56⁺ natural killer cells

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NKp80/KLRF1

NKp80, also known as killer cell lectin-like receptor subfamily F member 1 (KLRF1), is an 80 kDa type II transmembrane, homodimeric, activating co‑receptor expressed exclusively on the surface of NK cells. Human NKp80, a member of the C-type lectin family of proteins (1‑3), is 231 aa in length with a 38 amino acid (aa) cytoplasmic region, a 21 aa transmembrane region, and a 172 aa extracellular domain (ECD). The ECD contains the C-type lectin-like domain (aa 114‑230) and four potential sites of N-linked glycosylation. Multiple splicing variants produce four isoforms for NKp80. Isoform 1 is the standard protein. There are no murine orthologs for NKp80 (4). NKp80 functions as an inhibitory receptor based on the presence of two immunoreceptor tyrosine‑based inhibitory motifs (ITIM) in the N-terminal cytoplasmic region and the lack of charged amino acids in the transmembrane region (1). However, later studies show that NKp80 displays an activating function rather than an inhibitory one (2‑3, 5). In in vitro experiments in human NK cells, NKp80 stimulates NK cell cytotoxicity and induces calcium influx after triggering by appropriate antibodies (2, 3). One study reports that NKp80 likely functions as a co‑receptor, cooperating with NKp46 and other activating receptors in NK cell cytotoxicity (2). Activation-induced C-type lectin (AICL) is the ligand for NKp80 (3). It is a myeloid-specific receptor expressed by monocytes, macrophages, and granulocytes (3). Crosslinking of both NKp80 and AICL stimulates secretion of proinflammatory cytokines suggesting that NKp80‑AICL interaction is involved in the activating crosstalk between NK cells and myeloid cells, and thus may influence the initiation and maintenance of immune responses in humans (3).

References

Roda-Navarro, P. et al. (2000) Eur. J. Immunol. 30:568.
Vitale, M. et al. (2001) Eur. J. Immunol. 31:233.
Welte, S. et al. (2006) Nat. Immunol. 7:1334.
Yokoyama, W.M. and B.F. Plougastel (2003) Nat. Rev. Immunol. 3:304.
Thomas, M. et al. (2008) Proc. Natl. Acad. Sci. U.S.A. 105:1656.

Entrez Gene IDs

51348 (Human)

Alternate Names

Activating coreceptor NKp80; CLEC5C; CLEC5CMGC119908; C-type lectin domain family 5 member C; killer cell lectin-like receptor F1; killer cell lectin-like receptor subfamily F member 1; killer cell lectin-like receptor subfamily F, member 1; KLRF1; Lectin-like receptor F1; MGC119907; MGC119909; ML; NKp80

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human NKp80/KLRF1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Fully functional NK cells after unrelated cord blood transplantation.
Authors: Beziat V, Nguyen S, Lapusan S, Hervier B, Dhedin N, Bories D, Uzunov M, Boudifa A, Trebeden-Negre H, Norol F, Marjanovic Z, Marie JP, Vernant JP, Debre P, Rio B, Vieillard V
Leukemia, 2009-01-08;23(4):721-8.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
A novel subset of NK cells expressing high levels of inhibitory FcgammaRIIB modulating antibody-dependent function.
Authors: Dutertre CA, Bonnin-Gelize E, Pulford K, Bourel D, Fridman WH, Teillaud JL
J. Leukoc. Biol., 2008-08-21;84(6):1511-20.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry