Human NKp80/KLRF1 C-Terminus Antibody Summary
1% cross‑reactivity with recombinant human (rh) NKp44 and rhNKp30 is observed.
Val66-Tyr231
Accession # Q9NZS2
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: NKp80/KLRF1
NKp80, also known as killer cell lectin-like receptor subfamily F member 1 (KLRF1), is an 80 kDa type II transmembrane, homodimeric, activating co‑receptor expressed exclusively on the surface of NK cells. Human NKp80, a member of the C-type lectin family of proteins (1‑3), is 231 aa in length with a 38 amino acid (aa) cytoplasmic region, a 21 aa transmembrane region, and a 172 aa extracellular domain (ECD) (SwissProt # Q9NZS2). The ECD contains the C-type lectin-like domain (aa 114‑230) and four potential sites of N-linked glycosylation. Multiple splicing variants produce four isoforms for NKp80. Isoform 1 is the standard protein. There are no murine orthologs for NKp80 (4). One early study suggested that NKp80 functions as an inhibitory receptor based on the presence of two immunoreceptor
tyrosine‑based inhibitory motifs (ITIM) in the N-terminal cytoplasmic region, and the lack of charged amino acids in the transmembrane region (1). However, later studies show that NKp80 displays an activating function rather than an inhibitory one (2‑3, 5). In in vitro experiments in human NK cells, NKp80 stimulates NK cell cytotoxicity and induces calcium influx after triggering by appropriate antibodies (2, 3). One study reports that NKp80 likely functions as a co‑receptor, cooperating with NKp46 and other activating receptors in NK cell cytotoxicity (2). Activation-induced C-type lectin (AICL) is the ligand for NKp80 (3). It is a myeloid-specific receptor expressed by monocytes, macrophages, and granulocytes (3). Crosslinking of both NKp80 and AICL stimulates secretion of proinflammatory cytokines suggesting that NKp80‑AICL interaction is involved in the activating crosstalk between NK cells and myeloid cells, and thus may influence the initiation and maintenance of immune responses in humans (3).
- Roda-Navarro, P. et al. (2000) Eur. J. Immunol. 30:568.
- Vitale, M. et al. (2001) Eur. J. Immunol. 31:233.
- Welte, S. et al. (2006) Nat. Immunol. 7:1334.
- Yokoyama, W.M. and B.F. Plougastel (2003) Nat. Rev. Immunol. 3:304.
- Thomas, M. et al. (2008) Proc. Natl. Acad. Sci. U.S.A. 105:1656.
Product Datasheets
Citation for Human NKp80/KLRF1 C-Terminus Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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The Activating C-type Lectin-like Receptor NKp65 Signals through a Hemi-immunoreceptor Tyrosine-based Activation Motif (hemITAM) and Spleen Tyrosine Kinase (Syk)
Authors: B Bauer, T Wotapek, T Zöller, E Rutkowski, A Steinle
J. Biol. Chem, 2017-01-12;292(8):3213-3223.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
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