Human Nogo-A aa 566-748 Antibody Summary
Val566-Phe748
Accession # Q9NQC3
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
View Larger
Nogo‑A in Rat Cortical Stem Cells. Nogo-A was detected in immersion fixed 7 day differentiated rat cortical stem cells using 10 µg/mL Sheep Anti-Human Nogo-A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3515) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Nogo-A
Human Nogo-A (also reticulon-4) is a member of the reticulon family of transmembrane proteins. This family is characterized by the presence of a nonsignal sequence-containing N-terminus, a topologically conserved 200 amino acid (aa) C-terminus that contains two transmembrane domains with an ER-retention motif, and a punctate intracellular distribution within the ER that is reminescent of a reticulum (1-4). In human, Nogo exists in five isoforms (5-7). The full length human form (Nogo-A) is 1192 aa in length and contains a 1018 aa N-terminus, a 21 aa transmembrane segment, a 94 aa connecting “loop”, a second 21 aa transmembrane segment, and a 38 aa C-terminus. Three areas are of particular interest. One is a stretch of 66 aa within the 94 aa connecting loop. This segment is reported to bind to the GPI-linked Nogo receptor/p75 complex on axons and induce growth cone collapse (8-10). Two other areas in the N-terminus have also been discovered to have bioactivity (8, 11, 12). Based on rat, aa 57-184 in human (aa 59-172 in rat) should block fibroblast spreading, while aa 566-748 in human (aa 544-725 in rat) block neurite outgrowth and block fibroblast spreading (8, 12, 13). The exact topology of Nogo-A is unclear. The N- and C-termini may be extracellular with the “loop” region intracellular, or the situation could be reversed (13-15). Alternatively, the loop region and N-terminus may be on the same side of the membrane (3, 8). The four additional isoforms are shorter than Nogo-A (199 aa [Nogo-C], 373 aa [Nogo-B], 392 aa and 986 aa, respectively) (7). Although highly divergent, all contain the same C-terminal stretch, aa 1005-1192. Both Nogo-B and C are reported to complex with Nogo-A (16). Notably, Nogo-A is expressed in neurons, endothelial cells. oligodendrocytes, fibroblasts and myoblasts (12, 16-18). Human Nogo-A is 78% aa identical to mouse and rat Nogo-A overall, with 98% aa identity in the loop region and approximately 80% aa identity in the aa 566-748 segment.
- Oertle, T. et al. (2003) FASEB J. 17:1238.
- GrandPre, T. et al. (2000) Nature 403:439.
- Yan, R. et al. (2006) Cell. Mol. Life Sci. 63:877.
- Teng, F.Y.H. et al. (2004) J. Neurochem. 89:801.
- Chen, M.S. et al. (2000) Nature 403:434.
- Morris, N.J. et al. (1999) Biochim. Biophys. Acta 1450:68.
- GenBank Accession # Q9NQC3.
- Oertle, T. et al. (2003) J. Neurosci. 23:5393.
- Fournier, A.E. et al. (2001) Nature 409:341.
- Wang, K.C. et al. (2002) Nature 420:74.
- Prinjha, R. et al. (2000) Nature 403:384.
- Dodd, D.A. et al. (2005) J. Biol. Chem. 280:12494.
- Li, M. et al. (2004) Eur. J. Biochem. 271:3512.
- Huber, A.B. and M.E. Schwab (2000) Biol. Chem. 381:407.
- Ng, C.E.L. and B.L. Tang (2002) J. Neurosci. Res. 67:559.
- Dodd, D.A. et al. (2005) J. Biol. Chem. 280:12494.
- Wang, X. et al. (2002) J. Neurosci. 22:5505.
- Acevedo, L. et al. (2004) Nat. Med. 10:382.
Product Datasheets
Citations for Human Nogo-A aa 566-748 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
4
Citations: Showing 1 - 4
Filter your results:
Filter by:
-
Nogo receptor 1 regulates Caspr distribution at axo-glial units in the central nervous system
Authors: Jae Young Lee, Min Joung Kim, Lijun Li, Alexander A. Velumian, Pei Mun Aui, Michael G. Fehlings et al.
Scientific Reports
-
A proteolytic C-terminal fragment of Nogo-A (reticulon-4A) is released in exosomes and potently inhibits axon regeneration
Authors: Y Sekine, JA Lindborg, SM Strittmatt
Nat. Med., 2019-11-20;295(8):2175-2183.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Histamine Receptor 3 negatively regulates oligodendrocyte differentiation and remyelination
Authors: Y Chen, W Zhen, T Guo, Y Zhao, A Liu, JP Rubio, D Krull, JC Richardson, H Lu, R Wang
PLoS ONE, 2017-12-18;12(12):e0189380.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr -
Protein microarray analysis identifies cyclic nucleotide phosphodiesterase as an interactor of Nogo-A.
Authors: Sumiyoshi K, Obayashi S, Tabunoki H
Neuropathology, 2009-06-07;30(1):7-14.
Species: Mouse, Rat
Sample Types: Cell Lysates, Whole Tissue
Applications: IHC-P, Western Blot
FAQs
No product specific FAQs exist for this product, however you may
View all Antibody FAQsIsotype Controls
Reconstitution Buffers
Secondary Antibodies
Reviews for Human Nogo-A aa 566-748 Antibody
There are currently no reviews for this product. Be the first to review Human Nogo-A aa 566-748 Antibody and earn rewards!
Have you used Human Nogo-A aa 566-748 Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
