Human Nope/IGDCC4 Antibody

Catalog #: MAB10345 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB10345-100
MAB10345-SP

Detection of Human Nope/IGDCC4 by Western Blot.

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All Nope/IGDCC4 Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Nope/IGDCC4 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Nope/IGDCC4 in direct ELISAs.

Source

Recombinant Monoclonal Rabbit IgG Clone # 2516A

Purification

Protein A or G purified from cell culture supernatant

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human Nope/IGDCC4
Met1-Thr957
Accession # Q8TDY8

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Flow Cytometry

0.25 µg/10⁶ cells

See below

Immunohistochemistry

3-25 µg/mL

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Nope/IGDCC4 by Western Blot. Western blot shows lysates of human uterus tissue. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human Nope/IGDCC4 Monoclonal Antibody (Catalog # MAB10345) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Nope at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Flow Cytometry

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Detection of Nope/IGDCC4 in HEK293 Human Cell Line Transfected with Human NOPE and eGFP by Flow Cytometry HEK293 human embryonic kidney cell line transfected with either (A) human Nope or (B) irrelevant protein and eGFP was stained with Rabbit Anti-Human Nope/IGDCC4 Monoclonal Antibody (Catalog # MAB10345) followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). Quadrant markers were set based on control antibody staining (Catalog # MAB1050). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry

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Detection of Nope/IGDCC4 in A549 Human Cell Line by Flow Cytometry. A549 human cell line was stained with Rabbit Anti-Human Nope/IGDCC4 Monoclonal Antibody (Catalog # MAB10345, filled histogram) or Rabbit IgG control antibody (Catalog # MAB1050, open histogram) followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). View our protocol for Staining Membrane-associated Proteins.

Immunohistochemistry

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Nope/IGDCC4 in Human Placenta. Nope/IGDCC4 was detected in immersion fixed paraffin-embedded sections of human placenta tissue using Rabbit Anti-Human Nope/IGDCC4 Monoclonal Antibody (Catalog # MAB10345) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to syncytiotrophoblast. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Nope/IGDCC4

IGDCC4/Nope is a type I transmembrane protein that belongs to the immunoglobulin superfamily. It is expressed mostly in embryonic muscle tissues and in developing and adult nervous systems. The structural similarities between IGDCC4 and the guidance receptor of the DCC family suggest that IGDCC4 may have similar functions as the DCC family.

Long Name

Neighbor of Punc E11/Immunoglobulin Superfamily, DCC Subclass, Member 4

Entrez Gene IDs

57722 (Human); 56741 (Mouse)

Alternate Names

DDM36; hDDM36; IGDCC4; immunoglobulin superfamily DCC subclass member 4; immunoglobulin superfamily, DCC subclass, member 4; KIAA1628; likely ortholog of mouse neighbor of Punc E11; LOC57722; Neighbor of punc e11; Nope; NOPEFLJ42051; Protein DDM36