Human Notch-1 PE-conjugated Antibody Summary
Ala19-Gln526
Accession # P46531
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Notch‑1 in U2OS Human Cell Line by Flow Cytometry. U2OS human osteosarcoma cell line was stained with Mouse Anti-Human Notch-1 PE-conjugated Monoclonal Antibody (Catalog # FAB5317P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.
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Preparation and Storage
Background: Notch-1
Human Notch-1 is a 300 kDa type I transmembrane glycoprotein that is one of four human Notch homologues involved in developmental processes (1‑3). Notch signaling is important for maintaining stem cells and inducing differentiation, especially in the nervous system and lymphoid tissues (2‑4). Notch can specify binary cell fates; for example, promoting T‑ over B‑cell development from a common precursor (2). More than 50% of human T-lineage Acute Lymphoblastic Leukemia (T‑ALL) have activating mutations of Notch1 (1, 5). Human Notch-1 is synthesized as a 2556 amino acid (aa) precursor that contains an 18 aa signal sequence, a 1718 aa extracellular domain (ECD) with 36 EGF-like repeats and three Lin-12/Notch repeats (LNR), a 23 aa transmembrane (TM) segment and a 785 aa cytoplasmic domain containing six ankyrin repeats, a glutamine-rich domain and a PEST sequence. The 11th and 12th EGF-like repeats bind ligands including Jagged and Delta-like families in humans (6). O-fucosylation by Fringe family members at a site within this region can inhibit the interaction of Notch with Jagged ligands, thereby promoting Delta-like ligand interactions (7). Notch-1 receptor undergoes post-translational furin-type proteolytic cleavage, forming a heterodimer through interaction of a hydrophobic area C-terminal to the LNR on the 1647 aa ligand-binding extracellular region with the 891 aa transmembrane/cytoplasmic portion (8, 9). Upon ligand binding, additional sequential proteolysis by TNF-converting enzyme (ADAM-17) and the presenilin-dependent gamma -Secretase results in the release of the Notch intracellular domain (NICD) which translocates into the nucleus, activating transcription of Notch-responsive genes (10). Human Notch-1 ECD aa 19 - 526, including the first 13 EGF repeats, shows 91% aa identity with corresponding regions of mouse and rat, 89% with canine, and 79% with chicken Notch-1. This region also exhibits 60% aa identity with human Notch-2 and Notch-3.
- Ellisen, L. W. et al. (1991) Cell 66:649.
- Dumortier, A. et al. (2005) Int. J. Hematol. 82:277.
- Yoon, K. and N. Gaiano (2005) Nat. Neurosci. 8:709.
- Androutsellis-Theotokis, A. et al. (2006) Nature 442:823.
- Weng, A. P. et al. (2004) Science 306:269.
- Hambleton, S. et al. (2004) Structure 12:2173.
- Yang, L. et al. (2005) Mol. Biol. Cell 16:927.
- Sanchez-Irizarry, C. et al. (2004) Mol. Cell. Biol. 24:9265.
- Logeat, F. et al. (1998) Proc. Natl. Acad. Sci. USA 95:8108.
- Mumm, J.S. and R. Kopan (2000) Dev. Biol. 228:151.
Product Datasheets
Citations for Human Notch-1 PE-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 6
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Blockade of Oncogenic NOTCH1 with the SERCA Inhibitor CAD204520 in T Cell Acute Lymphoblastic Leukemia
Authors: Matteo Marchesini, Andrea Gherli, Anna Montanaro, Laura Patrizi, Claudia Sorrentino, Luca Pagliaro et al.
Cell Chemical Biology
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Notch Signaling Mediates Secondary Senescence
Authors: YV Teo, N Rattanavir, N Olova, A Salzano, A Quintanill, N Tarrats, C Kiourtis, M Müller, AR Green, PD Adams, JC Acosta, TG Bird, K Kirschner, N Neretti, T Chandra
Cell Rep, 2019-04-23;27(4):997-1007.e5.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Bepridil exhibits anti-leukemic activity associated with NOTCH1 pathway inhibition in chronic lymphocytic leukemia
Authors: S Baldoni, B Del Papa, E Dorillo, P Aureli, F De Falco, C Rompietti, D Sorcini, E Varasano, D Cecchini, T Zei, A Di Tommaso, E Rosati, G Alexe, G Roti, K Stegmaier, M Di Ianni, F Falzetti, P Sportolett
Int. J. Cancer, 2018-03-23;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Activation of Notch-1 in oral epithelial cells by P. gingivalis triggers the expression of the antimicrobial protein PLA2-IIA
Authors: A Al-Attar, Y Alimova, S Kirakodu, A Kozal, MJ Novak, AJ Stromberg, L Orraca, J Gonzalez-M, M Martinez, JL Ebersole, OA Gonzalez
Mucosal Immunol, 2018-03-07;11(4):1047-1059.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Leukemia-specific delivery of mutant NOTCH1 targeted therapy
Authors: Giovanni Roti, Jun Qi, Samuel Kitara, Marta Sanchez-Martin, Amy Saur Conway, Anthony C. Varca et al.
Journal of Experimental Medicine
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Blocking the NOTCH pathway inhibits vascular inflammation in large-vessel vasculitis.
Authors: Piggott K, Deng J, Warrington K, Younge B, Kubo JT, Desai M, Goronzy JJ, Weyand CM
Circulation, 2011-01-10;123(3):309-18.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
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So simple! Add the antibody (or its isotype control) to the cells (5uL for 1x10^6 cells), incubate for 30 minutes, wash and read!
