Human NPRB/NPR2 Antibody

Catalog #: MAB9725 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB9725-100
MAB9725-SP

Detection of NPRB/NPR2 in HEK293 cells transfected with Human NPR2 and eGFP by Flow Cytometry.

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All NPRB/NPR2 Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human NPRB/NPR2 Antibody Summary

Species Reactivity

Human

Specificity

Detects human NPRB/NPR2 in direct ELISA.

Source

Monoclonal Mouse IgG_2A Clone # 1060722

Purification

Protein A or G purified from cell culture supernatant

Immunogen

Mouse myeloma cell line, NS0-derived human NPRB/NPR2
Arg23-Ile458
Accession # P20594

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

HEK293 cells transfected with Human NPR2 and eGFP

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of NPRB/NPR2 in HEK293 cells transfected with Human NPR2 and eGFP by Flow Cytometry. HEK293 cells transfected with Human NPR2 and eGFP were stained with either (A) Mouse Anti-Human NPRB/NPR2 Monoclonal Antibody (Catalog # MAB9725) or (B) Mouse IgG_2A Isotype Control (Catalog # MAB003) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NPRB/NPR2

Human Natriuretic Peptide Receptor-2 (NPR2), also known as NPRB, ANP-RB or guanylyl Cyclase-B, is a member of the guanylyl cyclase family of proteins. NPR2 is a type I transmembrane glycoprotein that contains a 436 amino acid extracellular domain (ECD) (aa 23‑458) for ligand binding, and a 569 amino acid cytoplasmic domain that contains both a protein kinase domain and a carboxyl-terminal guanylate cyclase domain. NPR2 is expressed most highly in in bone, brain, fibroblasts, heart, kidney, liver, lung, uterine, and vascular smooth muscle tissue (1). NPR2 operates as an oligomer and binds both ANP (atrial natriuretic peptide) and BNP (B type natriuretic peptide), and NPR2 is the principal receptor of CNP (C type natriuretic peptide) (1, 2). Ligand binding to the extracellular ligand binding domain, plus ATP to the intracellular kinase domain activates a cytoplasmic guanylate cyclase (2). NPR2 pathway play a critical role in regulation of skeletal growth (3), and patients with single defect NPR2 alleles are statistically shorter than the average population (4). Over the extracellular domain, human NPR2 is 97% and 96% identical to mouse and rat NPR2, respectively.

References

Potter, L.R. et al. (2009) Handb Exp Pharmacol 191:341.
Chang, M.S. et al. (1989) Nature 341:68.
Tsuji, T. and Kunieda T. (2005) J Biol Chem 280:14288.
Olney, R.C. et al. (2006) J Clin Endocrinol Metab 91:1229.

Long Name

Natriuretic Peptide Receptor B

Entrez Gene IDs

4882 (Human); 230103 (Mouse); 116564 (Rat)

Alternate Names

AMD1;AMDM;ANPb;ANPRB;Atrial natriuretic peptide receptor 2;ECDM;GCB;GC-B;GUC2B;GUCY2B;NPRB;NPRBi;SNSK; AMDM; ANPb; ANPRB; ECDM; GC-B; GUC2B; NPR2; NPRB; NPRBi; SNSK