Human NQO-2 Antibody

Catalog #: AF7868 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF7868
AF7868-SP

Detection of Human NQO-2 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human NQO-2 Antibody Summary

Species Reactivity

Human

Specificity

Detects human NQO-2 in direct ELISAs and Western blots. In direct ELISAs, less than 3% cross-reactivity with recombinant human NQO-1 is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human NQO-2
Ala2-Gln231 (Leu47Phe)
Accession # P16083

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

0.25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human NQO-2 by Western Blot. Western blot shows lysates of human liver tissue, human kidney tissue, HepG2 human hepatocellular carcinoma cell line, K562 human chronic myelogenous leukemia cell line, and A549 human lung carcinoma cell line. PVDF membrane was probed with 0.25 µg/mL of Sheep Anti-Human NQO-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7868) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for NQO-2 at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NQO-2

NQO-2 (NADPH Quinone acceptor Oxidoreductase 2; also QR2, melatonin receptor 3/MT3 and NRH dehydrogenase) is a 25-26 kDa cytoplasmic flavoprotein member of the NAD(P)H dehydrogenase family of enzymes. It shows restricted expression, being found in retinal pigment epithelium, prostatic fibroblasts, select CNS neurons and RBCs. Like NQO-1, NQO-2 would appear to reduce catechol quinones, but without the use of NADH. In contrast to NQO-1, NQO-2 would appear to activate some quinones, generating cytotoxic products. NQO-2 also reportedly binds and stabilizes key cell protection molecules such as p53, thus acting as a gatekeeper for proteosome-mediated protein turnover. Finally, NQO-2 reportedly binds to melatonin, an act that may promote the subsequent binding of the androgen receptor and an inhibition of cell proliferation. Human NQO-2 is 231 amino acids (aa) in length. It contains a flavodoxin-like domain (aa 4-212) and three utilized Ser phosphorylation sites. NQO-2 functions as a noncovalent homodimer. There are two potential isoform variants. One shows an alternative start site at Met117, while another shows a deletion of aa 102-139. Full length human NQO-2 shares 81% aa sequence identity with mouse NQO-2, and less than 50% aa sequence identity with human NQO-1.

Long Name

NAD(P)H Dehydrogenase, Quinone 2

Entrez Gene IDs

4835 (Human); 18105 (Mouse); 291084 (Rat)

Alternate Names

DHQV; DIA6; dioxin-inducible-2; EC 1.10.99.2; NAD(P)H dehydrogenase, quinone 2; NAD(P)H menadione oxidoreductase 2, dioxin-inducible; NMOR2; NQO2; NQO-2; NRH dehydrogenase [quinone] 2; NRH:quinone oxidoreductase 2; QR2; Quinone Reductase 2; ribosyldihydronicotinamide dehydrogenase [quinone]; ribosyldihydronicotinamide dehydrogenase