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Human NTH1 Antibody

Catalog #: MAB2675
4 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB2675
MAB2675-SP
Detection of Human NTH1 by Western Blot.
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Product Details
Citations (4)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human NTH1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human NTH1 in Western blots.
Source
Monoclonal Mouse IgG1 Clone # 208521
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human NTH1
Met1-Leu312
Accession # P78549
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human NTH1 antibody by Western Blot. View Larger

Detection of Human NTH1 by Western Blot. Western blot shows lysates of DU145 human prostate carcinoma cell line and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Human NTH1 Monoclonal Antibody (Catalog # MAB2675) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF007). A specific band was detected for NTH1 at approximately 38 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NTH1

NTH1 is a DNA glycosylase with apurinic lyase activity. It functions in the repair of oxidative damage to DNA through the BER (base excision repair) pathway.

Long Name
Nth Endonuclease III-like 1
Entrez Gene IDs
4913 (Human)
Alternate Names
EC 4.2.99.18; endonuclease III-like protein 1; nth endonuclease III-like 1 (E. coli); NTH1; NTHL1; OCTS3; OCTS3NTH1nth (E.coli endonuclease III)-like 1

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Citations for Human NTH1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Identification of SUMO modification sites in the base excision repair protein, Ntg1
    Authors: Paul W Doetsch
    DNA Repair (Amst.), 2016-10-31;48(0):51-62.
    Species: Human
    Sample Types: Protein
    Applications: Western Blot
  2. Elevated FGF21 secretion, PGC-1alpha and ketogenic enzyme expression are hallmarks of iron-sulfur cluster depletion in human skeletal muscle.
    Authors: Crooks D, Natarajan T, Jeong S, Chen C, Park S, Huang H, Ghosh M, Tong W, Haller R, Wu C, Rouault T
    Hum Mol Genet, 2013-08-13;23(1):24-39.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  3. Both human ferredoxins 1 and 2 and ferredoxin reductase are important for iron-sulfur cluster biogenesis
    Authors: Yanbo Shi, Manik Ghosh, Gennadiy Kovtunovych, Daniel R. Crooks, Tracey A. Rouault
    Biochimica et Biophysica Acta (BBA) - Molecular Cell Research
  4. Altered expression of the human base excision repair gene NTH1 in gastric cancer.
    Authors: Goto M, Shinmura K, Igarashi H, Kobayashi M, Konno H, Yamada H, Iwaizumi M, Kageyama S, Tsuneyoshi T, Tsugane S, Sugimura H
    Carcinogenesis, 2009-05-04;30(8):1345-52.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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